Table of Contents
What is a Himar1 mariner transposon?
The Himar1 mariner transposon is one of the two known active mariner elements that have been successfully utilized for a number of oral and non-oral bacteria such as Pseudomonas aeruginosa (Withers et al., 2014), Porphyromonas gingivalis (Klein et al., 2012), and oral streptococci (Nilsson et al., 2014).
What is a transposon mutant?
Transposon mutagenesis, or transposition mutagenesis, is a biological process that allows genes to be transferred to a host organism’s chromosome, interrupting or modifying the function of an extant gene on the chromosome and causing mutation.
How many genes does Campylobacter jejuni have?
jejuni 81–176) through natural transformation. We identified 166 essential protein-coding genes and 20 essential transfer RNAs (tRNA) in C.
What is Tn5 transposon?
Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes.
How does Tn5 Tagmentation work?
Illumina developed the tagmentation protocol, in which a modified Tn5 enzyme cuts double-stranded DNA and concurrently ligates the linker sequences that are required for Illumina sequencing to both ends.
What are transposons give examples?
Transposons are the genetic elements associated with antibiotic resistance in certain bacteria. For instance, the Tn5 that codes for transposase Tn5 as well as those for antibiotic resistance to kanamycin.
What is an ez-tn5 transposon?
An EZ-Tn5 Transposon is any DNA sequence contained between the 19-bp Mosaic End (ME) sequence (shown in red) that is uniquely and speci˜cally recognized by EZ-Tn5 Transposase.
How do you store ez-tn5 transposase?
Storage:Store only at –20°C in a freezer with out a defrost cycle. Storage Buffer:EZ-Tn5 Transposase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, and 1 mM dithiothreitol.
What is the storage buffer for ez-tn5?
Storage Buffer:EZ-Tn5 Transposase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, and 1 mM dithiothreitol.