Table of Contents
What is Flag expression?
The FLAG® Expression System is an established way to express, purify and detect recombinant fusion proteins.
What does flag tag mean?
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine).
What is a FLAG tag antibody?
The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions.
What is a FLAG tag in Western blot?
What is the flag sequence?
Flag sequence: In data transmission or processing, a sequence of bits used to delimit, i.e. mark the beginning and end of a frame. Note 1: An 8-bit sequence is usually used as the flag sequence; for example, the 8-bit flag sequence 01111110.
How big is a FLAG tag?
about 1 kDa
FLAG is a short, 8 amino acid sequence DYKDDDDK about 1 kDa in size (D = aspartic acid, Y = tyrosine, K = lysine). Due to its small size, some researchers may choose to leave the tag intact, but cleavage can be performed with enterokinase.
What is the FLAG® tag?
The Flag® tag, also known as the DYKDDDDK-tag, is a popular protein tag that is commonly used in affinity chromatography and protein research for over 20 years now (6,7,8,9,10,11). As its second name suggests the tag consists of an amino acid sequence DYKDDDDK. (D=Aspartic acid; K=Lysine; Y=Tyrosine).
What is a bacterial expression vector?
From Wikipedia, the free encyclopedia A bacterial expression vector for expressing green fluorescent protein from the T7 promoter. An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells.
What is the role of the vector in gene expression?
The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector.
What is the peptide sequence of the FLAG-tag from the N-terminus?
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da).