What culture medium is used for Bordetella pertussis?
Diagnosis. B pertussis can be cultured on modified Bordet-Gengou medium, charcoal-horseblood agar (Regan-Lowe) or grown in supplement Stainer-Scholte broth.
Where does Bordetella pertussis bacteria live?
Pertussis, more commonly known as whooping cough, is caused by a bacterium (germ), Bordetella pertussis, that lives in the mouth, nose and throat. The germ is highly contagious and is easily spread from person-to-person.
Why is it difficult to grow Bordetella pertussis in a lab?
Bordetella organisms grow optimally at 35° to 37° C. Bordetella species are fastidious because their growth can be inhibited by components commonly found in laboratory media. In addition, their rate of growth is inversely related to their degree of fastidiousness.
Does Bordetella pertussis grow on MacConkey Agar?
B. pertussis does not grow on nutrient agar or MacConkey agar and grows poorly on blood agar. B. pertussis is weakly oxidase positive and is non-motile.
What type of pathogen is Bordetella pertussis?
Pertussis, a respiratory illness commonly known as whooping cough, is a very contagious disease caused by a type of bacteria called Bordetella pertussis. These bacteria attach to the cilia (tiny, hair-like extensions) that line part of the upper respiratory system.
What specimen of choice is used for Bordetella?
Nasopharyngeal swabs or aspirates are the specimens of choice for recovery of B.
Which of the following is the most suitable specimen for the isolation of Bordetella pertussis?
Nasopharyngeal secretions are the optimal specimens for isolating the pertussis bacterium and obtaining a lab confirmed diagnosis.
Does Bordetella pertussis grow on blood agar?
pertussis does not grow on nutrient agar or MacConkey agar and grows poorly on blood agar. B. pertussis is weakly oxidase positive and is non-motile.
Does Bordetella pertussis ferment lactose?
Bordetella avium These are non-lactose fermenting, small rods that are characterized by the ability to grow in aerobic conditions.
What agar is used to isolate Bordetella pertussis?
Remel Bordet Gengou Agar
Remel Bordet Gengou Agar, when enriched with blood, is a solid medium recommended for use in qualitative procedures for the isolation of Bordetella pertussis and Bordetella parapertussis. Whooping cough is caused by B. pertussis and B.
What bacteria causes Bordetella?
Is pertussis viral or bacterial?
Pertussis is also called whooping cough. It is a serious lung infection caused by bacteria. It is also very contagious and causes coughing fits.
How is Bordetella pertussis diagnosed?
By taking a nose and throat swab, they can test for the Bordetella pertussis bacteria in culture or a more rapid polymerase chain reaction test. Your doctor may suggest a blood test to check your white blood cell count, which, if low, signals the presence of an infection.
What bacteria causes pertussis?
Pertussis is also known as whooping cough. It is a highly infectious bacterial disease involving the respiratory tract. It is caused by a bacterium (Bordetella pertussis or Bordetella parapertussis ) that is found in the mouth, nose and throat of an infected person.
Is Bordetella pertussis aerobic or anaerobic?
B. pertussis is a strict aerobe and grows optimally at 35°C to 37°C 3 ; however, it is fastidious in its nutritional requirements as it specifically requires nicotinamide supplement 5 , and growth can be inhibited by the presence of fatty acids, metal ions, sulphides, and peroxides in the media 3 .