Table of Contents
What is Qubit for DNA?
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.
What is Qubit DNA quantification?
The Qubit quantification platform continues to evolve with you, offering: Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.
Does Qubit measure RNA?
Informational:Informational:New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.
How do you use Qubit?
1.5 Add 10 µL of each Qubit® standard to the appropriate tube, then mix by vortexing 2–3 seconds. Be careful not to create bubbles. Note: Careful pipetting is critical to ensure that exactly 10 µL of each Qubit® standard is added to 190 µL of Qubit® working solution.
What is a Qubit assay?
Invitrogen Qubit assays utilize target-selective dyes that emit fluorescence when bound to DNA, RNA or protein, unlike traditional UV absorbance, which can overestimate sample concentrations due to contaminants in the sample such as salts, solvents, detergents, proteins, free nucleotides.
Is Qubit or Nanodrop more accurate?
The sensitivity of the Qubit can be as low as 10 pg/mL, which is far superior to the Nanodrop. So, if you worry your samples have very low concentrations, it is best to use the Qubit. Another advantage the Qubit has over the Nanodrop is its specificity.
How does Qubit measure protein?
The Qubit Protein Assay Kit is designed specifically for use with any Qubit fluorometer. While using between 1 and 20 µL of sample, this assay can quantify samples ranging from 12.5 µg⁄mL to 5 mg⁄mL with low protein-to-protein variation.
Why is Qubit better than NanoDrop?
The main attraction for using a Qubit is when you have samples with very low concentrations of nucleic acids. The sensitivity of the Qubit can be as low as 10 pg/mL, which is far superior to the Nanodrop. So, if you worry your samples have very low concentrations, it is best to use the Qubit.
What does it mean if a DNA sample has a 260 280 ratio less than the minimum values in the range?
A 260/280 ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA. Abnormal 260/280 ratios usually indicate that a sample is contaminated by residual phenol, guanidine, or other reagent used in the extraction protocol, in which case the ratio is normally low.
Does qubit ssDNA work on single stranded DNA?
The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides.
Can I use the qubit ssDNA assay kit with my fluorometer?
The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but can be used with any fluorometer or fluorescence plate reader. The Qubit ssDNA kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA.
Does the Invitrogen qubit RNA BR and HS assay kits require DNase?
The Invitrogen Qubit RNA BR (broad range) and HS (high sensitivity) Assay Kits are highly selective for RNA over double-stranded DNA (dsDNA) and unlike other RNA assays do not require DNase if DNA is present in the sample for an accurate measurement.
Can the Qubit 4 measure RNA quality?
New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein. Achieve single-stranded DNA quantification using the Qubit ssDNA Assay Kit with any fluorometer or plate reader.