Table of Contents
Why is tubulin used as a control?
Nonetheless, β-actin and β-tubulin offer certain advantages as loading controls: they are highly conserved, display high expression level, and exhibit stability under most experimental conditions.
Is tubulin an antibody?
The α-Tubulin Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.
Is tubulin an antigen?
Abstract. The presence of circulating antibodies directed against a cytoskeletal element, microtubules, in patients with autoimmune thyroid disorders, has been studied using pure brain tubulin as antigen.
What is the molecular weight of beta tubulin?
55kDa
β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices.
What is the function of beta tubulin?
Highly conserved α- and β-tubulin heterodimers assemble into dynamic microtubules and perform multiple important cellular functions such as structural support, pathway for transport and force generation in cell division.
What does beta tubulin do?
The alpha beta tubulin heterodimer is the structural subunit of microtubules, which are cytoskeletal elements that are essential for intracellular transport and cell division in all eukaryotes.
What is the function of tubulin protein in g2 phase?
Tubulin is the protein that polymerizes into long chains or filaments that form microtubules, hollow fibers which serve as a skeletal system for living cells. Microtubules have the ability to shift through various formations which is what enables a cell to undergo mitosis or to regulate intracellular transport.
What is the function of the ß tubulin in the Western blotting experiments?
Beta-Tubulin, is usually used as loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What is alpha and beta tubulin?
Alpha and beta tubulins are the building blocks of microtubules, one of the three components of the cell cytoskeleton. As compared to the other proteins (microfilaments and intermediate filaments) that make up the cell cytoskeleton, microtubules are larger (in diameter).
What is alpha tubulin and beta tubulin?
Definition. Alpha tubulin refers to one of the two protein components of a microtubule attached to GTP while beta tubulin is the second protein component of a microtubule. Thus, this is the fundamental difference between alpha and beta tubulin.
What is the function of tubulin protein in G2 phase?
What is the reactivity of this antibody to beta tubulin?
This antibody reacts with the ~50 kDa beta-tubulin and has been shown to bind to the two major and one of the minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.
How is the β-tubulin antibody made?
The β-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.
What is the function of Beta III tubulin?
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance.
What is the predicted molecular weight of tubulin?
Predicted molecular weight: 55 kDa. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Ubiquitously expressed with highest levels in spleen, thymus and immature brain.