Table of Contents
How long is alteplase good for out of the fridge?
The solution may be used within 8 hours if stored at 2°C to 30°C (36°F-86°F).
How long is alteplase stable?
According to manufacturer’s data, reconstituted alteplase is stable for up to 8 hours, provided it is maintained at temperatures between 2°C and 30°C and at a concentration of 0.5 to 1 mg/mL.
How long does Activase alteplase remain stable at room temperature after reconstitution?
Conclusions: Alteplase diluted in normal saline solution at a concentration of 0.01 mg/mL is biochemically stable and active at ambient temperature for as long as 24 hours as assessed by in vitro clot lysis assays.
Can Cathflo be mixed with saline?
No interactions were found between Cathflo Activase and Normal Saline Flush. However, this does not necessarily mean no interactions exist. Always consult your healthcare provider.
Can alteplase be frozen?
Alteplase (1 mg/mL) reconstituted with sterile water and stored frozen for 8 days showed no statistical difference in enzyme activity between the individual modes of thawing (37°C, 20°C, and refrigerated).
How long is Cathflo good out of the refrigerator?
Cathflo Activase contains no antibacterial preservatives and should be reconstituted immediately before use. The solution may be used for intracatheter instillation within 8 hours following reconstitution when stored at 2-30°C (36-86°F). No other medication should be added to solutions containing Cathflo Activase.
How long is tPA good for after mixing?
According to the manufacturer, tPA can be used for 8 hours after reconstitution in sterile water at room temperature; this is a short period of time for laboratory use.
Can alteplase be filtered?
Subject: Cathflo® Activase® (alteplase) should be filtered with a 5 micron filter needle during withdrawal of the reconstituted solution to remove potential rubber stopper particulates.
What is the half-life of alteplase?
Alteplase converts plasminogen to the proteolytic enzyme plasmin, which lyses fibrin as well as fibrinogen. Intravenous alteplase is cleared primarily by the liver with an initial half-life of fewer than 5 minutes and a terminal half-life of 72 minutes.
Can you flush Cathflo?
If catheter function has been restored, ASPIRATE 4 to 5 mL of blood in patients ≥10 kg or 3 mL in patients <10 kg to remove Cathflo and residual clot. Then discard aspirate and flush the catheter with 0.9% Sodium Chloride, USP. Any unused solution should be discarded.
How do you reconstitute alteplase?
Reconstitution should be carried out using the transfer device provided and by adding the contents of the 100‑mL vial of preservative free SWFI to the 100-mg vial of Activase powder. Reconstitute Activase immediately before administration, using aseptic technique at all times.
What do you dilute tPA with?
Results in concentration of 1 mg/ml. May be infused at this concentration or may dilute further with equal volume of normal saline or D5W (0.5 mg/ml).
Does tPA need to be refrigerated?
How should I keep alteplase (TPA, Activase, Cathflo Activase) stored? The vials are stored at room temperature up to 80 F (30 C) or under refrigeration at 2-8 C (36-46 F). Once mixed with sterile water, it must be used immediately. Any drug not used must be thrown away.
Why can tPA be used after 3 hours?
Most of them are ineligible because they come to the hospital after the three-hour time window.” The timing of treatment is important, because giving a strong blood thinner like tPA during a stroke can cause bleeding inside the brain.
How long is tPA active?
The half-life of tPA in the bloodstream is rather short, 5-10 minutes in humans, as a result of PAI-1-mediated inhibition and LRP1-mediated liver uptake [14].
How long do you let Cathflo sit?
If function has not been restored, allow Cathflo to dwell for a total of 120 minutes. Then reassess function by once again attempting to aspirate blood. 06:14 If the catheter is still occluded, a second dose of equal amount may be instilled with 30 and 120 minute assessments done.
What is the half-life of Cathflo?
In patients with acute myocardial infarction administered 100 mg of Activase as an accelerated intravenous infusion over 90 minutes, plasma clearance occurred with an initial half-life of less than 5 minutes and a terminal half-life of 72 minutes.
Is tPA and alteplase the same?
Known by the generic name alteplase and marketed as Activase® (Genentech), tPA is given to patients through an IV in the arm, and it works by dissolving blood clots that block blood flow to the brain.
What is the stability of alteplase in normal saline?
Alteplase: stability and bioactivity after dilution in normal saline solution Alteplase diluted in normal saline solution at a concentration of 0.01 mg/mL is biochemically stable and active at ambient temperature for as long as 24 hours as assessed by in vitro clot lysis assays.
How long does alteplase last in the freezer?
Alteplase reconstituted and frozen in this manner has been shown to be stable and to retain bioactivity for up to 6 months ( 16,17 ). The frozen syringes were gently thawed at room temperature immediately prior to use.
What is alteplase used for?
Alteplase is a thrombolytic agent that is manufactured by recombinant DNA technology. It is FDA approved for use in acute ischemic stroke, pulmonary embolism, acute myocardial infarction, and occluded catheters.
What is the concentration of lyophilized alteplase?
Materials and methods: Two commercially available formulations of lyophilized alteplase (2-mg and 50-mg vials, respectively) were reconstituted with sterile water to a final concentration of 1 mg/mL.