Table of Contents
What are ISSR markers?
Inter Simple Sequence Repeats (ISSR) ISSR markers are highly polymorphic and are useful in studies on genetic diversity, phylogeny, gene tagging, genome mapping, and evolutionary biology (Reddy 2002). Sequences amplified by ISSR-PCR can be used for DNA fingerprinting.
Is ISSR a dominant marker?
ISSR markers are inherited in Mendelin mode and segregated as dominant markers. This technique has been widely used in the studies of cultivar identification, genetic mapping, gene tagging,genetic diversity, evolution and molecular ecology.
What are EST SSR markers?
Expressed sequence tag-derived simple sequence repeat markers (EST-SSRs) are the markers of choice, because they are abundant, co-dominant, highly polymorphic, and are easily transferable among phylogenetically related species [13].
Why is ISSR dominant?
ISSR markers are easy to use, low-cost, and methodologically less demanding compared to other dominant markers, making it an ideal genetic marker for beginners and for organisms whose genetic information is lacking.
What is PIC value of markers?
The Polymorphism Information Content (PIC) value is often used to measure the informativeness of a genetic marker for linkage studies. The PIC value was first derived for the case of a rare dominant disease, when one of the parents is affected, and is a function of the particular mode of disease inheritance.
Who discovered SSR markers?
Simple sequence repeats (SSRs) were discovered and developed by Litt and Luty (1989) and by Edwards et al. (1991) in humans and were first applied to plants by Akkaya et al. (1992). Genotyping technology: Specific loci are PCR amplified by specific primers flanking a simple repeat that consists of 1–5 nucleotides.
What is EST mapping?
EST (cDNA) mapping refers to the mapping of EST (cDNA) sequence file to the reference genome base sequence by using its homology to identify the genomic position from which EST is derived and registering the EST (cDNA) feature at that position.
What is SSR technique?
SSR genotyping involves the use of simple sequence repeats (SSRs) as DNA markers. SSRs, also called microsatellites, are a type of repetitive DNA sequence ubiquitous in most plant genomes. SSRs contain repeats of a motif sequence 1-6 bp in length.
What is EST analysis?
Abstract. Expressed sequence tags (ESTs) are fragments of mRNA sequences derived through single sequencing reactions performed on randomly selected clones from cDNA libraries. To date, over 45 million ESTs have been generated from over 1400 different species of eukaryotes.
What is the ISSR marker?
The ISSR markers use microsatellite sequences that are inherently highly variable and ubiquitously distributed across the genome, thus achieving higher reproducibility compared to other genetic markers (Jabbarzadeh et al. 2010, Costa et al. 2016). Tulipa suaveolens Roth is a decorative bulbous, polycarpic species in the Liliaceae family.
How much genetic variation do inter-simple sequence repeat (ISSR) markers generate?
The genetic variation within and among 30 populations was evaluated using inter-simple sequence repeat (ISSR) markers. Important findings: Twelve selected ISSR primers generated a total of 175 fragments, 173 (98.86%) of which were polymorphic.
What does ISSR stand for?
Inter-Simple Sequence Repeat (ISSR) markers: Are we doing it right? Inter-simple sequence repeats (ISSRs) are regions in the genome flanked by microsatellite sequences.
Are nonanchored ISSR markers a good choice for DNA fingerprinting?
Thus, nonanchored ISSR markers are a good choice for DNA fingerprinting. Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce.