Table of Contents
What are the advantages of elutriation method?
The practical advantages of elutriation include (1) the large numbers of cells that can be processed in a single run, (2) high recovery and viability, (3) rapidity, and (4) reproducibility. The equipment, once modified, can be easily operated and maintained in a clinical laboratory setting.
What is disadvantage in elutriation method?
One of the major disadvantages of centrifugal elutriation (CE) is the relatively large volume (150 ml) of the various fractions, especially if small numbers of cells have to be separated and the fractions contain few cells. To reduce the volume of the fractions 2 elutriator rotors were coupled in series.
What is elutriation which substance is purified by this method?
Elutriation is a process for separating particles based on their size, shape and density, using a stream of gas or liquid flowing in a direction usually opposite to the direction of sedimentation. This method is mainly used for particles smaller than 1 μm.
What is centrifugal elutriation?
Centrifugal elutriation is a velocity sedimentation method that separates cells on the basis of size, shape, and density. Lindahl first used this technique to separate cell subpopulations from a variety of biological systems (Lindahl 1956).
What is elutriation method?
Elutriation (the converse of sedimentation) is where a fluid (usually a gas) is forced through a powder bed, and can thus be used to determine particle size distributions. The basis of themethod is the ability of fluid velocities to support particles smaller than a given size.
What is elutriation tank?
Elutriation Tank: An elutriator is a straightforward device that divides particles into two or more groups. Elutriation occurs when a fluid moves in the opposite direction of particle sedimentation. Particle size, shape, and density all play a role in elutriation.
How does an Elutriator work?
Theory of Operation: An elutriator is essentially a vertical settling chamber. Elutriators consist of one or more vertical tubes or towers in series into which a dust-laden gas stream passes upward at a velocity defined by the gas flow rate and the tube cross-sectional area.