What is dynamic binding capacity?
Dynamic binding capacity is defined as the amount of product that will bind to the resin under typical flow conditions and must be determined under specific flow conditions and load characteristics [1], [2].
What is static binding capacity?
What is static binding capacity? The static binding capacity (SBC, also called total protein capacity) is normally measured in batch mode in a beaker. SBC is usually reported as the maximum amount of protein bound to a chromatography resin at given solvent and protein concentration conditions.
How does flow rate affect dynamic binding capacity?
DBC is dependent on the flow rate used during protein sample loading. For example, low flow rate gives longer residence time (the time the protein is in contact with the resin) and in the end higher DBC. For some resins, extended residence time is needed to utilize the full capacity.
What is Protein A resin?
Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens.
What is linear flow velocity?
In chromatography, flow rates should be standardized for columns of different dimensions by expressing the flow rate as linear flow velocity (cm/h). This is defined as the volumetric flow rate (cm3/h) per unit cross-sectional area (cm2) of a given column.
What is break through capacity?
Once the capacity reaches defined capacity value (defined by you), then time at that particular capacity or concentration is called breakthrough time.
What do binding proteins do?
A binding protein is any protein that acts as an agent to bind two or more molecules together. Most actin binding proteins bind on the actin surface, despite having different functions and structures.
What do you mean by continuity equation?
Continuity equation represents that the product of cross-sectional area of the pipe and the fluid speed at any point along the pipe is always constant. This product is equal to the volume flow per second or simply the flow rate. The continuity equation is given as: R = A v = constant.
How do you calculate linear flow rate?
Conversions between linear and volumetric flow rates can be done with the formula: Volumetric flow rate = (linear flow velocity/60) × column cross sectional area. F = (Y/60) × (πr2), where F = the volumetric flow rate (mL/min), Y = the linear flow velocity (cm/h), and r = the column inner radius (cm).
What is the difference between adsorption capacity and removal efficiency?
qe defines adsorption capacity i.e. unit weight of adsorbate (mg) adsorbent on per gram of adsorbent and is expressed as mg/g. Removal % defines the quantity of adsorbate removed in percentage (%) from a particular volume of adsorbate soluton.
What is moving bed adsorption?
A moving bed adsorption (MBA) process with heat integration is proposed as a potentially viable post-combustion process for the capture of CO2 from large-scale CO2-emitting plants. The proposed process consists of an adsorption bed and two desorption beds through which the adsorbent particles continuously circulate.
How is protein A virulence factor?
Staphylococcal protein A (SpA), a cell wall anchored protein of Staphylococcus aureus, has the ability to interact with several host components, possibly indicating a role as a virulence factor in S. aureus infections.