Is toluidine blue basic or acidic?
basic
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property.
What is the pH of P-toluidine?
5.3
The influence of pH on the permeability of p-toluidine (pKa, 5.3) and aminopyrine (pKa, 5.0) through shed snake skin as a model membrane was studied.
Is P-toluidine an acid?
p-Toluidine-2-sulfonic Acid.
Is toluidine blue a basic aniline dye?
Frequently, basic dyes (methylene blue, toluidine blue, thionine) will react with a specific tissue component and impart to it a color different from that of the dye itself. This phenomenon is called metachromasia and the cell or tissue components that exhibit it are said to be metachromatic.
What is the nature of P-toluidine?
colorless solid
P-toluidine is a colorless solid. Melting point 44°C (111°F). Specific gravity 1.046.
Why P-toluidine is more basic than M Toluidine?
It is correct that P-toluidine is a stronger base than m-toluene but reason is the hyperconjugation effect of methyl group from para position increases basic strength of para isomer.
Is methylene blue an acidic or basic dye?
Methylene blue (C.I. 52015; Basic blue 9) is a basic thiazine dye. It may have more scientific uses than any other dye. As a simple stain, applied from a mildly acidic solution (pH 3 to 4) it colors nucleic acids and acidic carbohydrates.
Why is toluidine blue used to stain cells?
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property.
What makes mast cells stain with toluidine blue?
Mast cells stain metachromatically with toluidine blue with greater intensity in cells containing smaller granules. Most stains for mast cells rely on the cell’s content of heparin, other glycosaminoglycans, and esterase.
Which stain contains acidic and basic dye?
Amphophilic – It is a term used to indicate that the tissue stains with both the basic and the acidic dyes.
Which is more basic O-toluidine or p-toluidine?
P-toluidine > m-toludine> aniline > 0-toluidine.
Which is more basic than para toluidine?
Assertion : p-nitroaniline is stronger base than p-toluidine . The electron withdrawing `NO_(2)` group in the p-nitroaniline makes it a stronger base .
Which one is more basic between benzylamine and para toluidine and why?
In aniline, p-methoxyaniline and p-methyl aniline, the lone pair of electrons on the N-atom is delocalised on the benzene ring while in benzylamine it is delocalised, and more available for donation. Hence benzylamine is most basic among the given.
Is methylene blue a pH indicator?
Tashiro’s indicator is a pH indicator (pH value: 4.4–6.2), mixed indicator composed of a solution of methylene blue (0.1%) and methyl red (0.03%) in ethanol or in methanol….Tashiro’s indicator.
| Tashiro’s indicator (pH indicator) | ||
|---|---|---|
| below pH 4.4 | above pH 6.2 | |
| 4.4 | ⇌ | 6.2 |
What is toluidine blue (TB)?
Toluidine blue (also known as tolonium chloride) is an acidophilic metachromatic dye that selectively stains acidic tissue components (sulfates, carboxylates, and phosphate radicals).[1] Toluidine blue (TB) has an affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high DNA and RNA content.[2]
What is toluidine blue used for in dentistry?
Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage.
When is toluidine blue and Lugol’s iodine used in the assessment?
Epstein JB, Scully C, Spinelli J. Toluidine blue and Lugol’s iodine application in the assessment of oral malignant disease and lesions at risk of malignancy. J Oral Pathol Med. 1992;21:160–3.
What is the utility of toluidine blue application in oropharyngeal carcinoma?
The utility of toluidine blue application as a diagnostic aid in patients previously treated for upper oropharyngeal carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod.