What are the different methods of haemoglobin estimation?
Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation but other methods like hemoglobin color scale, Sahli technique, Lovibond-Drabkin technique, Tallqvist technique, copper-sulfate method, HemoCue and automated haematology analyzers are also available.
Which method is used for estimation of Hb concentration?
Various methods are available for estimation of hemoglobin in the laboratory. The commonly used methods are Sahli’s/ acid hematin method and Cyanmethemoglobin method.
Why Cyanmethemoglobin method is the reference method for hemoglobin method?
The haemoglobin is estimated with the help of cyanmethemoglobin curve. The advantages of this method are i) error due to subjective visual matching is avoided as spectrophotometer is used and hence reading is precise and reliable, ii) measures all forms of haemoglobin except sulphaemoglobin.
What is hemoglobin cyanide method?
Cyanmethemoglobin Method The principle of this method lies in conversion of hemoglobin to cyanmethemoglobin by the addition of Potassium cyanide and ferricyanide whose absorbance is measured at 540 nm in a photoelectric calorimeter against a standard solution. The test was performed as outlined by Bhaskaram et al [12].
What is hemoglobin estimation?
Overview. A hemoglobin test measures the amount of hemoglobin in your blood. Hemoglobin is a protein in your red blood cells that carries oxygen to your body’s organs and tissues and transports carbon dioxide from your organs and tissues back to your lungs.
What are the haemoglobin estimation?
The healthy range for hemoglobin is: For men, 13.2 to 16.6 grams per deciliter. For women, 11.6 to 15 grams per deciliter.
Why is Cyanmethemoglobin is the best method?
Direct cyanmethaemoglobin method2 is the most widely used and recommended method by the International Committee for Standardization in Haematology for quantitative estimation of haemoglobin. It involves formation of a stable compound, cyanmethaemoglobin and is relatively a simple and cost-effective method3.
Why is Tallquist method no longer used?
The Tallquist method was included at first, but since blood hemoglobin values between 60 and 100 per cent could not be read with any degree of certainty, it was discarded as being inadequate for routine clinical work.
Why are the Tallquist’s and Dare’s methods no longer performed?
Why HCl is used in estimation of haemoglobin?
Answer. Answer: Explanation: The principle of Sahli’s Method or Acid hematin method is quite easy that when the blood is added to N/10 Hydrochloric acid (HCl), the hemoglobin present in RBCs is converted to acid hematin which is a dark brown colored compound.
What is the purpose of estimating haemoglobin?
Every clinician and surgeon recognizes the importance of hemoglobin estimation in affording valuable additional data in the diagnosis of certain diseases and conditions. By means of such blood examinations, the progress of the patient’s condition can be watched from time to time during the treatment of the disease.
Why is Tallquist method no longer performed?
Why HCl is used in Sahlis method?
A device called Sahli’s hemoglobinometer is used for this determination. The device contains hemoglobin tube, pipette, stirrer, and a comparator. Hydrochloric acid present in the device converts the hemoglobin into hematin acid which is then diluted so that the diluted hematin acid colour mathes with the comparator.
Is the Tallquist method accurate?
When compared to the Haemoglobin Cyanide method, the Tallquist method showed a calculated sensitivity of 96.6%, specificity of 87.4%, positive predictive value of 86.0%, and negative predictive value of 97.0% and diagnostic accuracy of 91.5%.
What is Tallquist method?
The Tallquist method of measuring haemoglobin is used to compare a small piece of Tallquist paper that has been saturated with a sample of blood with a Tallquist colour chart‏.