Table of Contents
What is Mono Q column?
Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.
What is Cex chromatography?
Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Cation exchange chromatography, more specifically, uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.
Why do we store chromatography columns in 20% ethanol?
Store your column in 20% ethanol Also, to prevent air from entering the column, connect the transport tool (provided with your new column) to the column outlet. Then, fill it with storage solution up to about 50% of its total volume.
How do you clean Monoq columns?
Clean the column after filter change according to regular cleaning. over the column 40 ml elution buffer at a flow rate of 2 ml/min through the column. Return to normal flow direction and run for 10 minutes at 4 ml/min. If high back- pressure persists, clean the column.
Why is salt used in ion exchange chromatography?
The Technique A salt gradient is used to elute separated proteins. At low salt concentrations, proteins having few charged groups are eluted and at higher salt concentrations, proteins with several charged groups are eluted. Unwanted proteins and impurities are removed by washing the column.
Is Q Sepharose a cation exchanger?
Q Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications. Well-proven strong anion exchanger developed for industrial downstream processes.
What is resin solution used for?
Prevent entry into waterways, sewers, basements or confined areas. A vapor-suppressing foam may be used to reduce vapors. Absorb or cover with dry earth, sand or other non-combustible material and transfer to containers.
How should columns be stored?
Columns should be stored at room temperature in their original box, with a copy of the certificate of analysis for future need and to evaluate the column prior to future use.
How do you remove silica from column chromatography?
Fine particle of silica can be tentatively eliminated by passing the product + small quantity of solvent into a pasteur pipette containing at the bottom a small plug of silanized glasswool. If the solvent volume is important , use several pipettes. Thank all of you very much.
Why does the pH matter for ion exchange chromatography?
The choice of buffer pH then determines the net charge of the protein of interest. In a buffer with a pH greater than the pI of the protein of interest, the protein will carry a net negative charge; therefore, a positively charged anion exchange resin is chosen to capture this protein.
Why choose resource q columns?
RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates. Quaternary ammonium (Q) strong anion exchanger for high resolution ion exchange chromatography. Mono-sized 15 µm rigid beads give low back pressure and high flow rates allowing for high productivity.
What is source 15q column used for?
RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates. Quaternary ammonium (Q) strong anion exchanger for high resolution ion exchange chromatography.
Why choose columns for chromatographic processes?
Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.
What is ion exchange chromatography (IEX)?
This ion exchange chromatography column generates low back pressures and high performance, making it excellent for ion exchange (IEX) chromatography on a large scale. As a member of the BioProcess resins range, SOURCE 15Q meets industrial demands with security of supply and comprehensive technical and regulatory support.