Table of Contents
What are the 129 Sv mice?
129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
What is a J mice?
Overview. The A/J strain is an inbred albino strain of mouse model. It is very frequently used in cancer research, on account of its strong tendency to develop tumors when presented with common carcinogens. It is one of the most popular strains used in mouse experiments.
How many mouse chromosomes are there?
20 chromosomes
Mice have 20 chromosomes in their haploid genome (thus 40 chromosomes in all). The haploid genome is about 3 picograms, similar to humans. The gene order of the genomes of mice and humans are conserved (synteny) although there are rearrangements, several per chromosome.
What are BALB c mice used for?
BALB/cJ mice are frequently used for a variety of immunological studies, in part because they demonstrate TH2-biased immune responses. BALB/c mice are particularly well known for the production of plasmacytoma on injection with mineral oil, forming the basis for the production of monoclonal antibodies.
What types of mice are used in research?
The survey results indicate that mouse is the overwhelmingly preferred laboratory animal; the most widely used mouse and rat strains are C57BL/6 mice, BALB/c mice, Sprague-Dawley rats and Wistar rats. Other strains, such as A/J mice, CD1 mice, and ICR mice, were also used.
How much DNA is in a mouse cell?
Surprisingly, only these 3 primer pairs, one from chromosome 10 and 2 from chromosome 9, demonstrated peak height ratios close to 23% (the amount of mouse DNA per cell is only about 85% that of human DNA per cell, so a 20% mixture by DNA amount converts to 22.9% mouse when considered as relative genomes).
What is the purpose of mice?
Mice are keystone species in almost every ecosystem. In forests, fields, and deserts, mice represent food to predators of all sizes. They link plants and predators in every terrestrial ecosystem. Weasels, foxes, coyotes, hawks, owls, skunks, shrews, bobcats, and bears all eat mice.
Why do we experiment on mice?
Even with differences between the two species, carrying out basic research in humanized mouse models of disease gives scientists valuable information. Using mice as surrogates allows researchers to first see how patients might respond to treatment before giving them the drug — a vital step in ensuring patient safety.
Are 1000 Genomes Phase 3 structural variants related to SV?
1000 Genomes Phase 3 structural variants as reported in a companion paper specifically dedicated to SV analysis. Much of these data are identical to those reported in the main paper as study estd214.
How is the 1000 genomes structural variation dataset built?
The 1000 Genomes Structural Variation dataset is built and validated on several different raw datasets. Rows with ‘*’ represent many differents archive runs, experiments and accessions. A full set or archive accessions can be found in the index files the FTP links point to.
Where will the VGP genomes be publicly available?
The VGP genomes will be publicly available on the Genome Ark. The Genome Ark is a database that is hosted by the Amazon cloud and integrated with existing international public genome browsing and analyses databases ( NCBI , ENSEMBL, and UCSC ), with our informatics and data management platform hosted by DNAnexus.