Table of Contents
What is the design of bioreactor?
Bioreactor design is a relatively complex engineering task, which is studied in the discipline of biochemical/bioprocess engineering. Under optimum conditions, the microorganisms or cells are able to perform their desired function with limited production of impurities.
What are the components of a typical bioreactor?
Different Parts of Bioreactor
- Fermenter Vessel/Vessel. The vessel is designed so that it uses the least work and maintains it and work is carried out in a clean manner under carefully controlled conditions.
- Heating and Cooling Apparatus.
- Sealing Assembly.
- Baffles.
- Impeller.
- Sparger.
- Feed Ports.
- Foam Control.
What is the basic design of fermenter?
Basic Design of a Fermenter The internal surface is smooth an corrosion resistant. Either stainless steel or glass is used for construction. Conventional bioreactors are cylindrical vessels with dome top and bottom (Figure 6.5). It is surrounded by a jacket and sparger at the bottom through which air is introduced.
What is bioreactor simple?
Bioreactor is defined as a vessel that carries out a biological reaction and is used to culture aerobic cells for conducting cellular or enzymatic immobilization.
What is difference between bioreactor and fermentor?
Bioreactor and fermentor are two words for basically the same thing. Scientists who cultivate bacteria, yeast, or fungi often use the term fermentor. The term bioreactor often relates to the cultivation of mammalian cells but is also generically used.
Which type of bioreactor mode is more efficient?
In general, the airlift bioreactors are more efficient than bubble columns, particularly for more denser suspensions of microorganisms. This is mainly because in these bioreactors, the mixing of the contents is better compared to bubble columns.
Is fermenter and bioreactor same?
What is tower bioreactor?
It is also known as a tower reactor. A bioreactor using an airlift system divides the fluid volume into two zones to improve circulation, oxygen transfer, and equalize forces in the reactor. In a two-zone system, only one zone is sparged with gas.
Is bioreactor or fermenter same?
What is bioreactor explain with diagram?
A bioreactor is a vessel in which raw materials are converted into products, using microbial plant, animal or human cells. It provides the optimal conditions by providing optimum temperature, pH, substrate, vitamins, oxygen, etc.
Why is pH important in bioreactor?
Controlling cell culture pH in bioreactors is critical to successful manufacturing. Most culture needs to be maintained in a specific narrow range of pH for maximum productivity. Typical pH range during bioproduction is 7.0± 1.0. Several factors affect pH including temperature, cell growth, lactic acid, and CO2 levels.
What is the highest temperature is used in bioreactors?
Most fermentations take place within the range 30 to 37°C; tight control of the temperature to within about 1°C is required. Because the metabolic activity of cells generates a substantial amount of heat, this heat must be removed to avoid temperature increases.
What is the ideal bioreactor design?
The ideal bioreactor design should maximize mass transfer to the hepatocytes hence maximizing the delivery of oxygen, nutrients, and toxins from patient’s blood to the hepatocytes. The treated blood or plasma is then returned to the patient.
What is the main function of a bioreactor?
The main function of a properly designed bioreactor is to provide a controlled environment to achieve optimal growth and/or product formation in the particular cell system employed. To design a bioreactor, some objectives have to be defined.
What are the different types of bioreactors?
Glass bioreactor (without a jacket) with an upper stainless steel lid. Glass bioreactor (with a jacket) with an upper stainless steel lid. Glass bioreactor (without a jacket) with the upper and lower stainless steel lids. Stainless steel bioreactor with peepholes. Ports for electrodes (pH, pO2, T, p).
What are single-use bioreactors made of?
Prior to the emergence of single-use bioreactor technology, bioreactors made from glass and stainless steel were the standards at bench scale and larger, respectively. However, single-use bioreactor systems are now commonplace in cell culture, with volumes from less than 500 mL–2000 L. Single-use fermentors are also available, from 25 to 500 L.