What is the excitation wavelength for Alexa 488?

What is the excitation wavelength for Alexa 488?

Alexa Fluor 488 is a fluorescent compound with an excitation peak at 499 nm and an emission peak at 520 nm. It can be excited using a 488 nm laser paired with a 530/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion.

What does Alexa Fluor do?

Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules.

What Colour is Cy3?

Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.

Is Cy3 red?

Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What is DyLight 488 and why is it essential?

DyLight® 488 is a green fluorophore commonly used in applications such as protein labeling, immunolabeling, fluorescence microscopy, and enzyme immunoassays. The dye has an absorption wavelength that peaks around 493 nm, and an emission maximum around 518 nm.

What does Cy3 stain for?

The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.

Does FITC bleed into PE?

Why do we need compensation? where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.

How do I choose Alexa Fluor?

Choose fluorophores with high quantum yields ( Φ ) A 100% efficient fluorescence process would have a quantum yield of 1 (the maximum quantum yield possible). The commonly used and very bright Alexa Fluor 488 fluorophore for example has a high quantum yield of 0.92 (Molecular Probes® 2010).

Can you freeze antibodies?

Most antibodies are stable for years when stored properly as per manufacturer’s recommendations. In most cases antibodies can be stored at -20 °C without any loss in their binding capacity. It is best to avoid storing antibodies in a frost-free freezer. This is to avoid or minimize freeze-thaw cycles.

What is maleimide derivative of Alexa Fluor 488?

The maleimide derivative of Alexa Fluor™ 488 is the most popular tool for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The resulting Alexa Fluor™ 488 conjugates exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.

What is Alexa Fluor 488 used for?

Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4 to pH 10.

How do you dissolve Alexa Fluor maleimide?

The Alexa Fluor® maleimide is typically dissolved in high-quality anhydrous dimethylsulfoxide (DMSO) at a concentration of 1-10 mM immediately prior to use, and stock solutions should be protected from light as much as possible.