Do plasma cells express B220?
Human plasma cells also express high levels of CD38, while mouse plasma cells also express Ly6k, Sca-1/Ly6, and CD98 and lose expression of B220/CD45 R.
Do plasma cells express CD138?
CD138 expression is a hallmark of plasma cells and multiple myeloma cells. However, decreased expression of CD138 is frequently observed in plasma cells of myeloma patients, although the clinical significance of this is unclear.
What does positive for CD138 mean?
Uses of CD138 Test. The specimens are observed under the microscope. If CD138 positive: Indicates Myeloma (plasma cell cancer) Or indicates Lymphoma (Lymphatic System Cancer) If CD138 negative: Usually indicates carcinomas (epithelial CE cancer) Directs towards other stains.
Do plasma cells express CD38?
Normal plasma cells show higher expression of CD38 than the hematopoietic stem cells, T and B lymphocytes. However, malignant plasma cells over-express CD38 at all stages of maturation.
What are B220 cells?
In reactive lymphoid tissues, B220 is expressed by B cells occupying the mantle zones and by a subpopulation of germinal center cells, but, in contrast, marginal zone B cells in the spleen do not express B220.
Where is CD38 expressed?
CD38 is expressed in many immune cell types, including bone marrow progenitors, natural killer cells, monocytes, and activated T- and B- lymphocytes [6].
What is CD138 negative plasma cells?
CD138 negative plasma cells are a sub-population of malignant plasma cells (PCs) which may be more primitive and have a higher proliferative potential than CD138 positive plasma cells [1].
What is CD138 in multiple myeloma?
Syndecan-1 or CD138, is a heparan-sulfate coated glycoprotein, which is highly expressed on the surface of plasma cells and myeloma cells, important for adhesion and accumulating survival signals.
Where is B220 found?
What is B220 Immunology?
B220 antigens represent a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker.
What does CD138 negative mean?
What is CD138 in bone marrow?
Syndecan-1 (CD138) mediates myeloma cell adhesion, and loss of syndecan-1 from the cell surface may contribute to myeloma proliferation and dissemination. Flow cytometry analysis of myeloma cells in bone marrow specimens shows heterogeneity in cell surface syndecan-1 expression.
What is the pathophysiology of B220+ and B220 (low) CD138 (+) plasma cells?
The numbers of B220(+) and B220(LOW) CD138(+) (plasma) cells increased sharply between days 4 and 7 post-infection (p.i.). At this time B220(+) cells expressed surface (s)IgM, but nearly all B220(LOW) CD138(+) cells showed concomitantly intracellular (i)IgM and IgG2a.
Can Blimp1 be detected in CD138 low population in bone marrow?
Here, we show in a Blimp1:GFP (green fluorescent protein) reporter mouse that the CD138 hi /B220 low population in bone marrow (BM) contains a significant fraction of Blimp1-negative cells. Therefore, detection of B220 and CD138 alone is not sufficient to identify PCs unambiguously.
What is CD138 and where is it expressed?
Its expression on cells of the B cell lineage correlates with their developmental stage, location, and adhesion. In mice, CD138 is expressed on pre-B and immature B lymphocytes in the bone marrow.
Do B220 cells express surface (S) IgM and intracellular (I) IgM?
At this time B220(+) cells expressed surface (s)IgM, but nearly all B220(LOW) CD138(+) cells showed concomitantly intracellular (i)IgM and IgG2a. Both follicular and marginal zone B cells were activated expressing high amounts of CD69.