What does Nile blue stain?

What does Nile blue stain?

Both Nile Blue and Nile Red fluoresce in the presence of lipids in multiple species. We have used both probes to stain cells from rodents, human cancer cell lines, marsupial cells and even planaria (data not shown).

What does Nile Red stain?

Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).

What is Nile blue test?

Nile blue staining Nile blue is used for histological staining of biological preparations. It highlights the distinction between neutral lipids (triglycerides, cholesteryl esters, steroids) which are stained pink and acids (fatty acids, chromolipids, phospholipids) which are stained blue.

Is Nile blue soluble in water?

Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose.

How do you make a Blue Nile?

(Method of Ostle and Holt, 1982) Prepare a 1% aqueous solution of the oxazine dye Nile blue A. Use mild heating to fully dissolve the dye if necessary. Filter the solution before use. Heat-fix smears of the cells and stain with the Nile blue A solution at 55°C for 10 min in a staining jar.

What is the pKa of Nile blue?

9.7
Nile blue is itself a pH indicator, but due to its pKa of 9.7 [28], there is barely any pH dependence in the physiological range, and additionally, it can be excited beside HPTS at 639 nm without any interaction between the two dyes. Hence, in this case, Nile blue acts as a pH reference dye.

What is Nile blue color?

Noun. Nile blue (countable and uncountable, plural Nile blues) A pale greenish blue colour.

What does BODIPY stain for?

With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.

What is Blue Nile liquid?

This liquid cleaner is a unique non-toxic solution formulated for the cleaning of diamonds, delicate gems (including pearls) and jewelry. A simple soak and light scrub with the included brush helps remove dirt and revive the shine and brilliance to your fine jewelry.

What is the density of Nile blue?

Predicted data is generated using the ACD/Labs Percepta Platform – PhysChem Module

What color is the Nile River?

The White Nile and Blue Nile derive their colors from the sediments they carry. Originating in the Equatorial Lakes region, the White Nile is rich in light gray sediments. As this long river meanders over flat terrain, it loses over half of its water to evaporation.

What does bodipy stain for?

Who is Nile red?

Against popular theories posed by NileRed’s subscribers, ‘Nile Red’ is not actually the name of the popular channel’s creator. Nigel Braun (pron. ‘brawn’) is the man behind the myth. The young Canadian chemist has been making videos since his early teens, but uploaded his very first YouTube video in March of 2014.

Why Blue Nile is called Blue?

Saifeddin says the name ‘Blue Nile’ was given to the River by Sudanese who call everything which is dark in color ‘Azraq(Blue). They called this Nile blue because of its dark turbid color due to the heavy silt it carries, in comparison with the White Nile that seems clear.