Table of Contents
What is fluorometer used for?
A fluorometer (and fluorimeter) measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength.
What is the principle of fluorometer?
Fluorophores absorb light of a distinct excitation wavelength and emit, or fluoresce, light of reduced energy thus a longer wavelength. This behavior can be modified so that the fluorescent reagents are restricted from emitting light unless bound to a specific molecule, such as dsDNA.
What are the components of a fluorometer?
Components of Fluorometers and Spectrophotofluorometers Instruments designed for fluorescence measurement have the following basic components: a light source, an excitation (primary) monochromator, a cuvette, an emission (secondary) monochromator, and a photodetector (Fig. 4.14).
What is difference between fluorescence and UV-Vis?
UV-Vis measures the absorption of light in this range, while fluorescence measures the light emitted by a sample in this range after absorbing light at a higher energy than it is emitting.
What is the best definition of fluorescence?
Definition of fluorescence : luminescence that is caused by the absorption of radiation at one wavelength followed by nearly immediate reradiation usually at a different wavelength and that ceases almost at once when the incident radiation stops also : the radiation emitted — compare phosphorescence.
What is the difference between fluorescence and absorbance?
Absorbance is measured as the difference in intensity between light passing through the reference and the sample, whereas fluorescence is measured directly without any reference beam. In other words, fluorescence is measured over a dark background, while absorbance is measured over a bright background.
What is fluorescence with example?
The emission of light from a material when subject to photons of another wavelength. A fluorescent object often emits visible light when it receives ultraviolet light. Fluorescence also occurs in nature; for example, fireflies and certain deep sea fish have fluorescent qualities.