Table of Contents
What is the principle of Microhematocrit method?
The microhematocrit method has the principle that the blood with anticoagulants is centrifuged at a certain speed, the erythrocytes will separate from the plasma. While the automated hematology analyzer uses the principle of flowcytometry which detects based on the height of the erythrocyte pulse.
What is Microhematocrit?
Medical Definition of microhematocrit 1 : a procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin. 2 : a hematocrit value obtained by microhematocrit a microhematocrit of 37 percent.
What are Microhematocrit used for?
Microhematocrit centrifuges are used for determination of volume fractions of erythrocytes (red blood cells) in blood and for separation of micro volumes of blood and solutions. It provides hematocrit values (HCT) for testing in biochemistry, immunity, genetics, blood separation and for general clinical tests.
What is Microhematocrit determination?
Microhematocrit method, n – The determination of the packed cell volume (PCV) using a small quantity of whole blood, a capillary tube, and a high-speed centrifuge. Packed cell volume, PCV, n – The measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood, expressed as a fraction.
How many Microhematocrit tubes are needed?
PROCEDURE – STEPWISE: Fill two mirohematocrit tubes from each EDTA blood tube to approximately 70-80% volume of tube. Total of four tubes. Keep tubes from one sample separate from the other. Stopper the tubes with clay, if necessary, and use a tissue to wipe excess blood from the microhematocrit tubes.
What are the precautions to be observed when performing a Microhematocrit?
What safety precautions should be observed when performing a microhematocrit? Observe Standard precautions; use plastic or Mylar-coated self-sealing tubes; close centrifuge lids securely; never open centrifuge until rotor has stopped; dispose of all sharps in appropriate containers.
What are advantages of the Microhematocrit test?
The measurement is useful in screening for anemia, screening potential blood donors, evaluating anemia therapies, and estimating blood loss following hemorrhage or trauma.
Which centrifuge used in Microhematocrit method?
The HemataStat II is a microhematocrit centrifuge system providing a quantitative hematocrit measurement. The hematocrit method measures the amount of red blood cells in relation to the amount of plasma. The test requires a small blood sample from a finger stick.
What are the advantages of Microhematocrit method over Macrohematocrit?
The micro-hematocrit method is more acceptable as it requires less amount of blood, and the testing time is very less in comparison to the macro-hematocrit method. Due to the requirement of less amount of blood and less time for the test, it may be useful in a mass survey.
How do you use a Microhematocrit centrifuge?
Procedure
- Fill two capillary tubes approximately 2/3 to 3/4 full with the well-mixed blood sample.
- Seal the dry end of the capillary tube by placing it into the sealing clay at a 90o angle.
- Place the capillary tubes in the microhematocrit centrifuge with the sealed end toward the periphery.
- Centrifuge for five minutes.
How do you use a Microhematocrit reader?
What are the disadvantages of the Microhematocrit?
The main disadvantage of the microhematocrit is that its measurement error increases at lower (anemic) values than that of the macrohematocrit method.
What are the advantages of Microhematocrit method?
What are the precautions when performing Microhematocrit?
What is microhematocrit and how is It measured?
Microhematocrit The reference method recommended by NCCLS of determining hematocrit or packed cell volume (PCV) is centrifugation. Hematocrit (PCV) is the measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood.
What is microhematocrit centrifugation?
microhematocrit centrifuge. an instrument that spins capillary tubes at a high speed to rapidly separate cellular components of the blood from the liquid portion of the blood. packed cell volume. the layers of blood cells that form when a tube of whole blood is centrifuged.
What is the reference method recommended by NCCLs for determining hematocrit?
The reference method recommended by NCCLS of determining hematocrit or packed cell volume (PCV) is centrifugation. Hematocrit (PCV) is the measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood.
How is hematocrit derived from the volume of passing erythrocytes?
The change in potential between the electrodes correlates to the volume of the passing erythrocyte. Furthermore, erythrocytes that have passed through the orifice are counted. From the mean cell volume, the erythrocyte count and the dilution factor, the hematocrit is derived.