Table of Contents
What is m-MLV Reverse Transcriptase?
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa.
What is M-MLV?
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
Do humans have a reverse transcriptase?
In cellular life Telomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
What is the difference between RNase A and RNase H enzymes?
The main difference between RNase A and RNase H is that the RNase A is specific for single-stranded RNAs, whereas RNase H is specific for RNA in a DNA: RNA duplex. Furthermore, RNase A produces 2′,3′-cyclic monophosphate intermediates while RNase H produces single-stranded RNA.
Does RNase H degrade DNA?
Nucleic Acid Immunity Furthermore, RNases H degrade RNA/DNA hybrids generated during viral replication.
What is M-MLV RT used for?
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa.
Where can I find Promega Helix products for my lab?
This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab’s needs. Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb).
What does M-MLV stand for?
RNA-Dependent DNA Polymerase for cDNA Synthesis Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa.
What is M-MLV reverse transcriptase?
This form of M-MLV Reverse Transcriptase has been genetically altered to remove the associated RNase H activity.