Table of Contents
How size exclusion chromatography works what is it used for?
Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. The gel consists of spherical beads containing pores of a specific size distribution. Separation occurs when molecules of different sizes are included or excluded from the pores within the matrix.
What Superdex 200?
Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification and also for characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as aggregate analysis of antibodies, size-homegeneity analysis of membrane proteins, or protein …
What does GPC measure?
An easy way to measure molecular weight of your sample is gel permeation chromatography (GPC). GPC is an analytical technique that separates molecules in polymers by size and provides the molecular weight distribution of a material. GPC is also sometimes known as size exclusion chromatography (SEC).
How many times a resin can be used?
To avoid performance loss during protein A chromatography cycling, manufacturers often use sodium hydroxide for cleaning as it has the ability to hydrolyze proteinaceous residues and simultaneously sanitize the resin [6-8] yet the maximum number of times a protein A resin can be reused remains variable, ranging from 50 …
What is the purpose of size exclusion chromatography?
Size exclusion chromatography (SEC) has been the main chromatographic method used to determine the size of proteins, and in particular the distribution of aggregates in final DP. The chromatographic matrix consists of beads with pores of a defined size, which allows for penetration of protein molecules.
What does Sephadex G 100 mean?
Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography. It has been used: in gel filtration column for α-amylase purification.