Table of Contents
What is size exclusion chromatography best used for?
Size exclusion chromatography (SEC) is the most common and efficient method to obtain information about the molecular weight and molecular weight distribution of macromolecules.
Where is size exclusion chromatography used?
Size exclusion chromatography (SEC), also known as gel filtration chromatography, is a chromatographic method that separates molecules based on their particle size (actually particle’s Stokes radius). It is usually applied to large molecules or macromolecular complexes such as proteins and polymers.
How do you clean a Superdex 200 column?
Cleaning is performed at low flow rates using 1 CV of sodium hydroxide (500 mM sodium hydroxide for Superdex and Superose columns and 200 mM sodium hydroxide for Sephacryl columns). Note that the column should never be stored in sodium hydroxide.
What is the most common support material for a packed GC column?
Diatomaceous earth
Explanation: Diatomaceous earth is the commonly used support material for the packed column in gas chromatography. The columns could be made of glass or metal.
How Long Does Size Exclusion chromatography take?
Due to the difference in size of two polymers with identical molecular weights, the absolute determination methods are, in general, more desirable. A typical SEC system can quickly (in about half an hour) give polymer chemists information on the size and polydispersity of the sample.
What is a Superdex 75?
Superdex® 75 10/300 GL is a prepacked gel filtration column for high-resolution, semipreparative, and analytical separations of biomolecules. Superdex® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000.
What is selection of chromatography systems from GE Healthcare?
Selection of chromatography systems from GE Healthcare suitable for size exclusion chromatography, buffer exchange, or desalting. 114 18-1022-18 AL Appendix 5 Converting from linear flow (cm/h) to volumetric flow rates (ml/min) and vice versa
What is size exclusion chromatography?
What is size exclusion chromatography? Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques. SEC separates molecules by differences in size as they pass through a resin packed in a column.
What is the purpose of desalting in size exclusion chromatography?
Desalting and other types of buffer exchange are examples of group separations on size exclusion chromatography (SEC) resins. The purpose is to separate small molecules, such as salts, from large biomolecules such as proteins. Samples can be prepared for storage or for other chromatography techniques and assays.
What is the difference between IEX and SEC chromatography?
Introduction SEC separates molecules according to differences in size as they pass through a SEC medium packed in a column. Unlike IEX or AC, molecules do not bind to the chromatography medium so buffer composition does not directly affect resolution (the degree of separation between peaks).