What is IgG and IgM in chikungunya?
IgM and IgG Positive: -IgM and IgG antibodies to Chikungunya virus detected, suggesting recent or past infection. IgM antibodies to Chikungunya virus may remain detectable for 3 to 4 months post-infection. IgM Positive, IgG Negative: -IgM antibodies to Chikungunya virus detected, suggesting recent infection.
What are the diagnosis test for chikungunya and dengue fever?
Diagnostic Tests for Suspected Chikungunya and Dengue During the first five days of illness, RT-PCR to directly detect CHIKV or DENV nucleic acid should be performed on serum from suspected cases.
Which test is best for chikungunya?
Several methods can be used for diagnosis of chikungunya virus infection. Serological tests, such as enzyme-linked immunosorbent assays (ELISA), may confirm the presence of IgM and IgG anti-chikungunya antibodies. IgM antibody levels are highest 3 to 5 weeks after the onset of illness and persist for about 2 months.
What tests determine chikungunya?
Detection of chikungunya virus (CHIKV) or viral RNA is the primary laboratory test used to diagnose infection in serum collected <6 days after onset of illness. Two real-time reverse transcription–polymerase chain reaction (RT-PCR) kits are available commercially, but validity data are limited.
What is the normal range of chikungunya?
Reference Range: Chikungunya Antibody, IgG 0.79 Index or less Negative: No significant level of Chikungunya IgG antibody detected. 0.80-1.09 Index Equivocal: Questionable presence of Chikungunya IgG antibody detected. Repeat testing in 10-14 days may be helpful.
Can Chikungunya cause low WBC count?
Chikungunya, being a viral infection, causes leucopenia or lymphopenia. Only one of our cases presented with low WBC count. The same patient also had thrombocytopenia, which is unusual for Chikungunya (Table 1).
How is Chikungunya diagnosed?
Laboratory diagnosis is generally accomplished by testing serum or plasma to detect virus, viral nucleic acid, or virus-specific immunoglobulin (Ig) M and neutralizing antibodies.
What is dengue serology test?
What is the test? The dengue MAC-ELISA is used for the qualitative detection of dengue virus IgM antibodies. The MAC-ELISA is based on capturing human IgM antibodies on a microtiter plate using anti-human-IgM antibody followed by the addition of dengue virus antigens.
How is chikungunya diagnosed?
Can chikungunya cause anemia?
Rheumatoid factor was reported positive in around 8–13% of chikungunya cases [7]. Followup investigations implied massive continuing inflammation, such as anemia with thrombocytosis, and elevated erythrocyte sedimentation rate (ESR).
Is chikungunya viral or bacterial?
Chikungunya (chik-un-GUN-yuh) is a viral illness transmitted by mosquitoes that causes the sudden onset of fever and severe joint pain. Other signs and symptoms may include fatigue, muscle pain, headache and rash.
Which tests are used to diagnose Chikungunya virus (CHIKV)?
Detection of chikungunya virus (CHIKV) or viral RNA is the primary laboratory test used to diagnose infection in serum collected <6 days after onset of illness. Two real-time reverse transcription–polymerase chain reaction (RT-PCR) kits are available commercially, but validity data are limited.
When should Chikungunya virus infection be considered in patients with polyarthralgia?
Chikungunya virus infection should be considered in patients with acute onset of fever and polyarthralgia, especially travelers who recently returned from areas with known virus transmission.
What is the sensitivity of the Realstar chikungunya RT-PCR kit?
Performance of the RealStar Chikungunya RT-PCR Kit (Altona Diagnostics, Hamburg, Germany) was assessed by Panning et al in the premarket format and was found to have a sensitivity (approximately 5.3 RNA copies/ reaction) and specificity comparable to that of the in-house assay [16].
Which real-time RT-PCR assays are used in the CDC’s evaluation of CHIKV IgM?
The CDC also provides CHIKV RNA–positive controls and primer/probe nucleotide sequences for real-time RT-PCR testing. Nine of 10 commercial CHIKV IgM detection assays were evaluated at the CDC, but only the Inbios and Euroimmun MAC-ELISAs, the Euroimmun IIFT, and the reformatted Abcam MAC-ELISA had acceptable performance.