Table of Contents
Which antisera is used in determining blood?
The Anti-A, Anti-B, and Anti-A,B reagents are used in the red blood cell determination of the ABO blood group. They are used to determine the absence or presence of erythrocytic antigens A and/or B on the surface of human red blood cells.
How many types of antiserum are used in blood group?
The Anti-D reagents: Anti-D, Anti-D (PK 1), Anti-D (PK 2), are used to determine the Rh(D) type. They are used to detect the presence of the Rh(D) antigen on the surface of human red blood cells. The Anti-C, Anti-E, Anti-c, Anti-e, and Anti-K are used for Rh Subgroups and Kell phenotyping of human red blood cells.
What are the examples of antisera?
Antiserums are produced in animals (e.g., horse, sheep, ox, rabbit) and man in response to infection, intoxication, or vaccination and may be used in another individual to confer immunity to a specific disease or to treat bites or stings of venomous animals.
What is the Colour of antisera D?
Anti-A, Anti-B, Anti-A,B and Anti-D blood grouping reagents are provided in a final buffered gel suspension. Anti-A has been colored with FD & C Blue #1 and Anti-B has been colored with FD & C Yellow #5. Anti D is a blend of monoclonal human IgM secreted by mouse/human hybridomas.
When are antisera used?
Antisera are used to determine which antigens are on the red cells. The test using antisera and the patient’s red cells is called the front cell group.
What is the function of Anti-D sera?
Anti-D is routinely and effectively used to prevent hemolytic disease of the fetus and newborn (HDFN) caused by the antibody response to the D antigen on fetal RBCs. Anti-D is a polyclonal IgG product purified from the plasma of D-alloimmunized individuals. The mechanism of anti-D has not been fully elucidated.
Which Colour is anti-A?
The colour of this antiserum is imparted by the dyes in which the blue colour of anti-A is due to the trypan blue. The anti-A is the serum from agglutination reaction with the antigen A.
What is the Colour of antisera B?
The Anti-B reagent is for the in vitro detection and identification of the B antigen on human red blood cells by direct agglutination. The formulation also contains sodium chloride and EDTA and 1g/l sodium azide. The reagent is coloured yellow with tartrazine.
What is anti serum used for?
Antiserum is a blood serum containing monoclonal or polyclonal antibodies that is used to spread passive immunity to many diseases via blood donation (plasmapheresis).
What are the Colours of antisera?
Alternatively, monoclonal antisera can be prepared from cultured cell lines. The government regulations specify that anti-A be coloured blue as a quality control (QC) measure. The government regulations specify that anti-B be coloured yellow as a QC measure. Anti-A,B is colourless (no dye is added).