How do you calculate RNA concentration?
Concentration of original RNA sample = 40 x A260 x dilution factor = 40 x 0.23 x 50. RNA concentration: 460 µg/ml. Total yield = concentration x volume of sample (ml) = 460 µg/ml x 0.1 ml.
How are Katals calculated?
1 katal = amount of enzyme that catalyzes the conversion of 1 mole of substrate per second (1 katal = 1 mol/s). Thus, 1 Unit = 1.667 × 10-8 katal and 1 katal = 6.00 × 107 Units.
What is the DNA concentration μg μl of a 50 μl solution which contains 10 μl of DNA at a concentration of 4 μg μl?
Answer: There are two ways to solve this problem: Calculate the total amount of DNA in the solution, then divide by the total volume: 10 µl x 4 µg/µl = 40 µg of DNA. 40 µg DNA/ 50 µl = 0.8 µg/µl.
How do you calculate resuspension?
To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add. The equation on the left shows an example, assuming you have an oligonucleotide of 1 nmol final yield.
How do you convert concentration to OD?
conversion factors:
- 1 OD260 Unit = 50 μg/ml for dsDNA.
- 1 OD260 Unit = 40 μg/ml ssRNA.
- 1 OD260 Unit = 33 μg/ml ssDNA.
How do you convert enzyme units to concentration?
Enzyme Activity 1 — Enzyme Units
- Enzyme units are expressed as µmol substrate converted per min.
- If the question gives enzyme activity in nmol per min, divide by 1000 to convert to µmol.
- Then multiply by the volume to get the total number of units.
How do you find the concentration of a stock solution?
The calculator uses the formula M1V1 = M2V2 where “1” represents the concentrated conditions (i.e., stock solution molarity and volume) and “2” represents the diluted conditions (i.e., desired volume and molarity).
How do you calculate ug from Ng uL?
ng/uL↔ug/mL 1 ng/uL = 1 ug/mL.
How do I make a 50 ug mL solution?
So convert 100 mg/mL into ug/mL = 100,000 ug/mL. You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL. You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
How do you find concentration after reconstitution?
The reconstitution calculator evaluates the volume and mass required to reconstruct a drug by adding a liquid diluent to a dry ingredient….
- Note down the mass of the dry ingredient.
- Note down the volume of the diluent.
- Divide the mass by volume.
- The result is reconstitution concentration.
Why do the ratios of 260 280 and 260 230 reflect the purity of RNA?
Note: RNA will typically have a higher 260/280 ratio due to the higher ratio of Uracil compared to that of Thymine. This ratio is used as a secondary measure of nucleic acid purity. The 260/230 values for “pure” nucleic acid are often higher than the respective 260/280 values.
How do you calculate RNA concentration from OD 260?
After measuring the optical densities the concentration of RNA can be calculated as follows: [RNA] (μg/ml) = 40 x Dilution Factor x OD260.
How do you calculate units of enzyme in ML?
Enzyme units are expressed as µmol substrate converted per min. If the question gives enzyme activity in nmol per min, divide by 1000 to convert to µmol. Then multiply by the volume to get the total number of units.
How do you calculate total enzyme concentration?
The enzyme has a molecular mass of 50,000 g/mole, so we calculate the enzyme concentration to be (0.01 g/L) / (50,000 g/mole) = 2 x 10-7 mol/L = 0.2 µmoles/L. Now we divide Vmax by the enzyme concentration: (3 µmoles/L/min) / (0.2 µmoles/L) = 15 min-1 = 0.25 s-1.
What is the recommended concentration of RNase A?
Used in conjunction with RNase T1 Recommended concentration of RNase A is 1 to 100 µg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids.
How do you calculate A260 RNA concentration?
The nucleic acid concentration is calculated using the Beer-Lambert law, which predicts a linear change in absorbance with concentration (Figure 1). Using this equation, an A260 reading of 1.0 is equivalent to ~40 µg/ml single-stranded RNA.The A260/A280 ratio is used to assess RNA purity.
How can I prepare RNase A at a lower pH?
prepare a 10 mg/mL stock solution in 10 mM sodium acetate buffer, pH 5.2. then adjust to pH 7.4 using 0.1 volume of 1 M Tris-HCl, pH 7.4. If RNase A is boiled at a neutral pH, precipitation will occur. When boiled at the lower pH, some precipitation may occur because of protein impurities that are present.
What is the recommended concentration of ribonuclease A for protection assay?
• Ribonuclease protection assays. Used in conjunction with RNase T1 Recommended concentration of RNase A is 1 to 100 µg/mL depending on the application. The enzyme is active under a wide range of reaction conditions.