What is the role of EF-Tu?

What is the role of EF-Tu?

Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function.

What is the role of EF-Tu during bacterial translation?

In prokaryotes, the primary function of EF-Tu is to transport the correct aa-tRNA to the A-site of the ribosome. As a G-protein, it uses GTP to facilitate its function.

What does EF-Tu and EF-G do?

EF-Tu brings aminoacyl-tRNA to the ribosomal A-site and its GTPase activity is stimulated by recognition of the mRNA codon by the aminoacyl-tRNA anticodon at the decoding centre of the small subunit. EF-G acts after completion of the peptidyl transferase reaction on the large subunit.

What is EF-Tu GTP?

Elongation factor Tu (EF-Tu, EF1-alpha) is a GTP-binding protein that is responsible for carrying each aminoacyl-tRNA to the ribosome, a process that involves hydrolysis of GTP and dissociation of the complex.

Where does EF-Tu bind to tRNA?

the ribosome
The binding site for translation factors Elongation factor Tu (EF-Tu or EF1α in eukaryotes) binds to the ribosome as a ternary complex with an aminoacyl-tRNA and GTP. The factor-binding site is just inside the L12 stalk of the large subunit with some contacts to the small subunit.

Does EF-Tu bind F met tRNA?

Tu will not form a binding complex with fMet-tRNA, preventing it from binding to the A site.

What is the role of EF-G?

Elongation factor G (EF-G) uses energy stored in GTP to catalyze movement of transfer RNAs and messenger RNA in the ribosome during the translocation step of prokaryotic protein synthesis.

Does EF-Tu bind a GTP?

Does EF-Tu hydrolyze GTP?

Abstract. In each round of ribosomal translation, the translational GTPase elongation factor Tu (EF-Tu) delivers a transfer RNA (tRNA) to the ribosome. After successful decoding, EF-Tu hydrolyzes GTP, which triggers a conformational change that ultimately results in the release of the tRNA from EF-Tu.

Where does EF Tu bind to tRNA?

Does EF Tu hydrolyze GTP?

Is EF-Tu a GTPase?

Among the GTPase translation factors, EF-Tu has an essential function in tRNA selection during decoding. EF-Tu in the GTP-bound form has high affinity for aminoacyl tRNAs (aa-tRNAs). The ternary complex of EF-Tu, aa-tRNA and GTP binds to the ribosomal A-site in the initial step of decoding.

Is EF-G A protein?

EF-G on the ribosome L7/L12 is only a multicopy protein on the large ribosomal subunit of the bacterial ribosome that binds to certain GTPases, like Initiation Factor 2, Elongation factor-Tu, Release Factor 3, and EF-G.

What are the antibiotics targeting EF-Tu?

Antibiotics targeting EF-Tu can be categorized into one of two groups, depending on the mechanism of action, and one of four structural families. The first group includes the antibiotics pulvomycin and GE2270A, and inhibits the formation of the ternary complex.

What is the difference between EF-1A and EF-Tu?

As a family of elongation factors, EF-Tu also includes its eukaryotic and archaeal homolog, the alpha subunit of eEF-1 (EF-1A). Elongation factors are part of the mechanism that synthesizes new proteins through translation in the ribosome.

What is the function of tRNA and EF Tu?

EF-Tu (blue) complexed with tRNA (red) and GTP (yellow) EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor responsible for catalyzing the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome.

What does efef tu stand for?

EF-Tu ( elongation factor thermo unstable) is a prokaryotic elongation factor responsible for catalyzing the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. It is a G-protein, and facilitates the selection and binding of an aa-tRNA to the A-site of the ribosome.