What is FITC and TRITC?
FITC and TRITC are among the most simple and commonly used reagents for protein fluorescent labeling. These isothiocyanates will crosslink to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups on a protein. However, only the derivatives of primary and secondary amines generally yield stable products.
What wavelength is TRITC?
TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.
Is Cy3 a TRITC?
Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.
Is Cy3 red or green?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
What color is TRITC?
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
Is TRITC cell permeable?
TRITC-dextrans are primarily used for studying permeability and transport in cells and tissues (5). An added benefit is that measurements of the fluorescence provide quantitative data on transport and permeability of healthy and diseased tissues.
What does TRITC stain for?
Tetramethylrhodamine (TRITC) dashboard Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.
What is TRITC filter?
What does Tritc stain for?
What does FITC stand for?
Fluorescein isothiocyanate
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
What is FITC in flow cytometry?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
What color is Tritc?
What are cyanine dyes used for?
Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques (Ünlü et al., 1997; Osterman et al., 2013) [5,6].