What is Polyethylenimine group?
Polyethylenimine (PEI) or polyaziridine is a polymer with repeating units composed of the amine group and two carbon aliphatic CH2CH2 spacers. Linear polyethyleneimines contain all secondary amines, in contrast to branched PEIs which contain primary, secondary and tertiary amino groups.
How do you resuspend someone on PEI?
This is what I do:
- Stir the PEI in MQ (1mg/ml) and dissolve by heating to ~80 degrees C. Vortex intermittently.
- Cool to RT and then pH with 1M Hepes to final concentration of 15mM.
- Filter through 0.22um filter –> aliquot –> freeze at -20 degrees C. Once thawed a working tube of PEI can be kept at 4 degrees C.
Is Polyethylenimine safe?
Ingestion Toxic if swallowed. Skin May be harmful if absorbed through skin. May cause skin irritation. Eyes May cause eye irritation.
Is PEI toxic to cells?
2), the PEI itself can exert significant cellular toxicity so that the IC50 for LPEI and BPEI respectively were 74 mg and 37 mg (Fig. 3). To evaluate transfection efficiency and safety of BPEI for gene delivery, Yoon et al.
Is Polyethylenimine toxic?
Introduction. Polyethylenimine (PEI), as a nonviral cationic polymer, has been widely used as gene delivery nanosystem. Although a number of investigations have highlighted its toxic impacts on target cells through induction of apoptosis/necrosis, still it is essential to look at its structural impacts on target cells.
How do you make Polyethylenimine?
Preparation of PEI Stocks
- Dissolve 100 mg in 100 mL sterile ddH2O.
- Stir while slowly adding HCl to pH 7.0.
- Mix for 10 minutes and then recheck pH.
- Filter sterilize through 0.22 um filter.
- Aliquot 500 uL to 1000 uL and store in -80C.
- Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed.
What is PEI DNA?
Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther.
Is Polyethylenimine biodegradable?
Polyethylenimine (PEI) is a class of cationic polymers proven to be effective for gene delivery. However, PEI is nondegradable and the molecular weight of PEI affects the cytotoxicity and gene transfer activity.
Is Polyethylenimine water soluble?
Polyethyleneimine (PEI) is a cationic water-soluble polymer with abundant amine groups. It was reported that PEI was used as a dispersing agent to improve the dispersity of LFP in its aqueous slurry [12] and as water-soluble binder for silicon anode [13].
What is PEI biology?
Poly(ethylenimine) (PEI) is an amino-rich highly hydrophilic cationic polyelectrolyte that has also been used as a functionalizing agent for carbon materials.
How do you make PEI?
What is nitrogen to phosphate ratio?
This was later supported by hundreds of independent measurements. However, looking at the composition of individual species of phytoplankton grown under nitrogen or phosphorus limitation shows that this nitrogen to phosphorus ratio can vary anywhere from 6:1 to 60:1.
What is PEI made of?
PEI is produced via the polycondensation reaction between bisphenol-A dianhydride such as tetracarboxylic dianhydride (produced from the reaction of bisphenol A and phthalic anhydride) and a diamine such as m-phenylene diamine. The early laboratory process involved a costly and difficult synthesis.
How do you calculate nitrogen phosphorus ratio?
The simplest method of determining a ratio is to divide the weights of nitrogen, phosphate, and potash by the lowest weight of the three.