What does a Zetasizer measure?
Zetasizer range Instruments in the Zetasizer family are used to measure the particle size of dispersed systems from sub-nanometer to several micrometers in diameter, using the technique of Dynamic Light Scattering (DLS).
How do I export raw data from zetasizer?
Data can be exported using File → Export. Chose Export to file, either all (everything in the list) or selected, and browse to your folder. On the Parameters tab, select Use export template parameters and select the appropriate template.
How does a Zetasizer measure zeta potential?
The charge or zeta potential of particles and molecules is determined by measuring their velocity while they are moving due to electrophoresis. Particles and molecules that have a zeta potential will migrate towards an electrode if a field is applied.
What is PDI in zetasizer?
The polydispersity index (PDI) is a number calculated from a simple two parameter fit to the correlation data called a cumulants analysis. The Polydispersity index is dimensionless and scaled such that values smaller than 0.05 are rarely seen other than with latex standards.
What is Z average in zetasizer?
The Z average is the intensity weighted mean hydrodynamic size of the ensemble collection of particles measured by dynamic light scattering (DLS).
How do I clean my Zeta cuvette?
To clean the cuvette, use a Luer-lock syringe to rinse with 3 washes of the same solvent used for the measurement using a 30-ml syringe followed by 3 washes of Milli-Q water. Dry with by expressing air through the cell with the same 30-cc syringe. Only use this cuvette prior to talking to the tool managers.
What is a good PDI value?
The numerical value of PDI ranges from 0.0 (for a perfectly uniform sample with respect to the particle size) to 1.0 (for a highly polydisperse sample with multiple particle size populations). Values of 0.2 and below are most commonly deemed acceptable in practice for polymer-based nanoparticle materials [82].
How do you calculate PDI?
The formula for polydispersity index is presented as Eq. (10.2). Where, PDI=the square of the standard deviation divided by the mean particle diameter. For example, nanoparticles with a Z-average value of 100 nm with PDI of 0.1 would have a standard deviation of 31.6 nm.
What does PDI mean in DLS?
Polydispersity Index
As it is a moments expansion it can produce a number of values, however only the first two terms are used in practice, a mean value for the size (Z-Average), and a width parameter known as the Polydispersity Index (PdI).
What is PDI width?
1.2 The Polydispersity Index. The polydispersity index (PDI) is another important parameter that describes the width or spread of the particle size distribution.
How do I clean my Zeta cell?
Cleaning the cuvettes To clean the cuvette, use a Luer-lock syringe to rinse with 3 washes of the same solvent used for the measurement using a 30-ml syringe followed by 3 washes of Milli-Q water. Dry with by expressing air through the cell with the same 30-cc syringe.
What is the proper procedure for cleaning the cuvettes?
Cleaning the Cuvette: Rinse the tube with distilled water a few times. Add about 1 mL of the solution to be measured. Tilt and turn the cuvette so that the solution has contact with all the surfaces. Discard this solution and repeat this rinse once more.