How do you Analyse a GC report?

How do you Analyse a GC report?

How to Read GC/MS Chromatograms

1. The X-Axis: Retention Time. Usually, the x-axis of the gas chromatogram shows the amount of time taken for the analytes to pass through the column and reach the mass spectrometer detector.
2. The Y-Axis: Concentration or Intensity Counts.
3. Differences in Gas Chromatogram Models.

What can you analyze with gas chromatography?

What is gas chromatography? Gas chromatography (GC) is an analytical technique used to separate the chemical components of a sample mixture and then detect them to determine their presence or absence and/or how much is present. These chemical components are usually organic molecules or gases.

What is GCMs analysis?

Gas Chromatography/Mass Spectrometry, or GC/MS analysis, is an analytical method that combines the features of gas chromatography and mass spectrometry to identify different substances within a sample component matrix. GC/MS analysis is generally considered one of the most accurate analyses available.

How do you prepare a sample for GC analysis?

Solid GC Samples Add one or two “specks” of solid16 (a pile approximately 2mm in diameter), or a very small spatula-tip of solid to a GC vial (Figure 2.93c). Then add a low boiling solid (e.g. methanol, clean acetone, diethyl ether, or dichloromethane) to the 1.5mL mark.

How a chromatogram is analyzed in gas chromatography?

In gas chromatography, the components of a sample are dissolved in a solvent and vaporized in order to separate the analytes by distributing the sample between two phases: a stationary phase and a mobile phase.

How do you read MS results?

How to Read a Simple Mass Spectrum

1. Step 1: Step 1: Identify the Molecular Ion.
2. Step 2: Step 2: Identify Major Fragmentation Clusters.
3. Step 3: Step 3: Determine the ∆m for Each Major Peak.
4. Step 4: Step 4: Identify Any Heteroatoms.
5. Step 5: Step 5: Identify Remainder of Molecule.
6. Step 6: Step 6: Name the Molecule.

How do you prepare samples for GC-MS analysis?

GC-MS sample preparation protocols

1. Filtration/Dilute and shoot.
2. Protein precipitation.
3. Phospholipid depletion.
4. Non-selective SPE (hydrophobic only)
5. Liquid-liquid extraction.
6. C18 silica based SPE.
7. C8 silica based SPE.
8. C2 silica based SPE.

How do you write a gas chromatography sample?

In general, SPE involves four steps:

1. Column preparation or prewash step.
2. Sample loading or the retention of the analytes of interest on the cartridge and/or sorbent.
3. Column postwash to remove undesirable contaminants.
4. Analyte desorption from the cartridge.

What types of samples are ideal for GC?

Organic or inorganic; abundant or in trace amounts; in solid, liquid, or gas form—most sample types and reasonably-volatile analytes can be analyzed using a choice of gas chromatography (GC) (link to GC overview page) approaches. Outstanding results and flexibility do not require great expense or complexity.

How do you identify components in a GC analysis?

A GC/MS system scans the samples continuously throughout the separation and, ultimately, the chromatogram shows the retention times. The chromatogram peak is analyzed by the mass spectrometer which leads to the identification of the separated components.

What is CAS in GC-MS?

The CAS number is just a database index and it is probably out of date as a way of identifying compounds. One difficulty is that a given chemical entity can have several numbers (depending for example on the salt form).