What does RNA-binding protein do?
RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay.
What is HuR biology?
The ubiquitous RNA-binding protein (RBP) HuR is one of the best-studied regulators of cytoplasmic mRNA fate. Through its post-transcriptional influence on specific target mRNAs, HuR can alter the cellular response to proliferative, stress, apoptotic, differentiation, senescence, inflammatory and immune stimuli.
How do RNA-binding proteins bind RNA?
To understand RBP regulation of RNA targets one must understand the biochemical underpinnings that facilitate exact and specific interaction with these sites. RNA binding proteins (RBPs) bind their RNA targets through the molecular interactions of chemical moieties between protein residues and RNA nucleotides.
Is Mrna an element?
The adenylate uridylate-rich elements (AREs) mediate the rapid turnover of mRNAs encoding proteins that regulate cellular growth and body response to exogenous agents such as microbes, inflammatory and environmental stimuli. However, the full repertoire of ARE-containing mRNAs is unknown.
How do RNA-binding proteins bind?
To understand RBP regulation of RNA targets, one must understand the biochemical underpinnings that facilitate exact and specific interaction with these sites. RBPs bind their RNA targets through the molecular interactions of chemical moieties between protein residues and RNA nucleotides.
Are AU rich element?
The AU-rich element (ARE) is one of the major cis-regulatory elements in the 3′ untranslated region (UTR) of labile mRNAs. Many of these encode factors requiring very tight regulation, such as inflammatory cytokines and growth factors.
How RNA-binding proteins interact with RNA molecules and mechanisms?
How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms. RNA-binding proteins (RBPs) comprise a large class of over 2,000 proteins that interact with transcripts in all manner of RNA-driven processes. The structures and mechanisms that RBPs use to bind and regulate RNA are incredibly diverse.
Why is siRNA important?
One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference).
How do you identify RNA-binding proteins?
Abstract. Regulatory RNAs exert their cellular functions through RNA-binding proteins (RBPs). Identifying RNA-protein interactions is therefore key for a molecular understanding of regulatory RNAs. To date, RNA-bound proteins have been identified primarily through RNA purification followed by mass spectrometry.