What is SuperScript VILO?
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes.
Why is cDNA used for PCR?
Quantitative reverse transcription PCR (RT-qPCR) is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.
How does oligo dT work?
Oligo(dT) primers amplify only mRNAs containing a poly(A) tail, since that is where the primer binds to promote reverse transcription. Random primers amplify most RNA species, including degraded RNA and viral genomes.
What viruses use reverse transcriptase?
Reverse transcriptases are used by viruses such as HIV and hepatitis B to replicate their genomes, by retrotransposon mobile genetic elements to proliferate within the host genome, and by eukaryotic cells to extend the telomeres at the ends of their linear chromosomes.
Are cDNA and mRNA the same?
Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.
What is superscript Vilo master mix?
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations.
What is superscript Vilo cDNA synthesis?
The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.
What is the lunascript RT Supermix master mix?
Request a sample LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures.
What is superscript™IV Vilo no RT control?
The SuperScript™IV VILO No RT Control contains all the components of the SuperScript™IV VILO Master Mix except the reverse transcriptase enzyme. The No RT Control is used in a RT minus reaction to verify the absence of genomic DNA (gDNA) contamination in the RNA sample.