When performing ionic Chromatography analysis the Eluent Generator allows?
Eluent generation allows the automatic production of high purity ion chromatography (IC) eluents. This is made possible through precise control of the electric current applied to the electrolysis of water to generate hydroxide and hydronium ions.
What is the purpose of eluent?
In an ion-exchange separation, the role of the eluent is to compete with solute ions for the fixed ions on the stationary phase and to separate the mixture of solute ions into well-defined bands.
What substances are used as eluent?
Another suitable eluent for anion chromatography is a solution containing boric acid and a polyol, gluconic acid. These substances combine to give acids that are stronger than boric acid. At pH 8.5, the usual pH for borate–gluconate eluents, most of the acids are ionized and the eluting power is adequate.
What is another name for the eluent?
The eluent or eluant is the “carrier” portion of the mobile phase. It moves the analytes through the chromatograph. In liquid chromatography, the eluent is the liquid solvent; in gas chromatography, it is the carrier gas.
Who invented ion exchange chromatography?
In order to separate proteins an ion exchange chromatographic method was reported by Peterson and Sober in 1956. In modern form ion exchange chromatography was introduced by Small, Stevens and Bauman in 1975 [3].
What is a good eluent?
A two-solvent eluent should contain one polar solvent and one non-polar solvent; BUT it is important that these two solvents are miscible with (i.e., dissolve in) each other. A good bet is to start with a ratio of 1:1, and then adjust the ratio depending on results obtained from developed chromatograms.
What is the difference between eluate and eluent?
What is the difference between Eluent and Eluate? Eluent is the portion of the mobile phase, which carries the sample components with it. Eluate is the combination of the mobile phase and the analytes.
How do I choose an eluent?
If you want the Rf of your TLC spot to be smaller, i.e., the spot to be lower down on the plate, you must decrease the eluent polarity. Either choose a different eluent (solvent) or adjust the solvent ratio by increasing the percentage of the nonpolar solvent relative to the polar solvent in the eluent.
Is mobile phase the eluent?
The eluent is the mobile phase or the solvent that is passed through the column. Molecules in the sample will desorb off the adsorbent and dissolve in the eluent when the polarity of the eluent matches the polarity of the molecules.
What is eluent in HPLC?
Eluent. The eluent or eluant is the “carrier” portion of the mobile phase. It moves the analytes through the chromatograph. In liquid chromatography, the eluent is the liquid solvent; in gas chromatography, it is the carrier gas.
Is eluent the same as mobile phase?
Eluent is the portion of the mobile phase, which carries the sample components with it. In liquid chromatography, eluent is the solvent used as the mobile phase.
What is reagent free IC eluent generation (RFIC-EG)?
Systems with Reagent-Free IC Eluent Generation (RFIC-EG) automatically generate high-purity hydroxide, carbonate, bicarbonate or methanesulfonic acid (MSA) eluent electrolytically using Thermo Scientific Dionex EGC Eluent Generator Cartridges. All that’s needed on an RFIC-EG system is a high-purity source of deionized water.
What is a carbonate EGC eluent generator cartridge?
Dionex Carbonate EGC Eluent Generator Cartridges for Anion Analysis Generate potassium carbonate eluent electrolytically. This cartridge is used with the Thermo Scientific Dionex Electrolytic pH Modifier for anion separations with carbonate eluent based columns.
How do I purify eluent using the Thermo Scientific Dionex CR-TC?
Eluent is purified on-line using the Thermo Scientific Dionex CR-TC Continuously Regenerated Trap Column, and suppressed electrolytically before detection, without the need to prepare regenerant. * The Thermo Scientific Dionex RFC-30 controller can be used to enable RFIC-EG on other Dionex IC systems.
What is an eluent cartridge?
Eluent generation (EG) cartridges electrolytically produce high-purity hydroxide, carbonate, and methanesulfonic acid eluents for isocratic and gradient runs in RFIC systems. Just add water. Automate generation of potassium, sodium and lithium hydroxide eluents.