Table of Contents
How many loci are amplified during the Identifiler plus PCR?
15 loci
The combination of the 15 loci are consistent with several worldwide database recommendations. The AmpFlSTR Identifiler Plus Kit delivers a 16-locus multiplex with the same power of discrimination as, better sensitivity than, and better robustness than the earlier generation of the AmpFlSTR Identifiler Kit.
What is Identifiler?
The AmpFLSTR Identifiler PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems. The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards.
What is the difference between GlobalFiler and PowerPlex 21?
There are two main differences between the two individual kits. The first difference is that GlobalFiler™ and GlobalFiler™ Express utilizes a 6-dye chemistry while the PowerPlex® Fusion™ and Fusion™ Direct utilizes a 5-dye kit. While one kit is a 6-dye and one kit is a 5-dye, the same number (24) loci are called.
What is Identifiler plus?
Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence.
What PowerPlex 21?
The PowerPlex® 21 System is a multiplex STR system for human identification applications including forensic analysis, relationship testing and research use.
What DNA profiling kit is used for PCR amplification?
YFiler Plus PCR Amplification Kit The Yfiler Plus kit is approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
What is a 13 loci DNA profile?
Within the U.S., the 13-STR profile is a widely used means of identification, and this technology is now routinely employed to identify human remains, to establish or exclude paternity, or to match a suspect to a crime scene sample.
How rare is a DNA profile?
The probability for a chance match, between any two unrelated individuals’ DNA profiles, is one in trillions with SGMPlus, though the possibility of a chance match between very common loci is greater.
What are the odds of a DNA match?
2d 460, 1995), the calculated probability of a match between suspect and evidence DNA was one in 19 billion. Since the denominator is more than three times the population of the earth, should this genotype be regarded as unique?
How many loci are in the identifiler kit?
The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards. • Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube. • Loci consistent with all major worldwide STR standards.
How many STR and amelogenin loci are in identifiler?
The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards. • Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube.
What is the locus designation for ampf™LStR identifiler™allelic ladder dye?
Locus designation Chromosome location Alleles included in AmpF™lSTR Identifiler™Allelic Ladder Dye label Control DNA 9947A D8S1179 8 8, 9 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 6-FAM™13†
What is the pI value of the identifiler kit?
5Probability of paternity exclusion The PIvalue is the probability that two individuals selected at random will have an identical Identifiler™Kit genotype (Sensabaugh, 1982). The PI values for the populations described in this section are then approximately 1/7.64 x 1017