What is ex527?
Selisistat (EX 527, SEN0014196) is a potent and selective SIRT1 inhibitor with IC50 of 38 nM in a cell-free assay, exhibits >200-fold selectivity against SIRT2 and SIRT3.
Does nicotinamide inhibit SIRT1?
Cell Mol Life Sci. 2017 Sep;74(18):3347-3362. doi: 10.1007/s00018-017-2527-8.
What is the role of sirtuins?
Sirtuins are NAD+-dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell metabolism, and caloric restriction.
How do I increase my SIRT1 activity?
The enzymatic reaction catalyzed by Sirt1 In this scenario, a low-energy status would increase Sirt1 activity by increasing the levels of NAD+, whereas a high-energy status would decrease Sirt1 activity by decreasing the levels of NAD+ [2]. An ample body of research has largely found this to be the case.
What is sirtuin pathway?
Sirtuin signaling pathway cascade. SIRT1 in the liver primarily regulates gluconeogenesis, glycolysis, insulin sensitivity, fatty acid oxidation, and cholesterol metabolism. In the event of excess energy, the liver can store energy by synthesizing glycogen and forming lipid droplets.
What protein does the SIRT1 gene encode?
Sirtuin 1 (SIRT1) which is encoded by the SIRT1 gene is the most conserved mammalian nicotinamide adenine dinucleotide (NAD+) dependent histone deacetylase (1). Besides its role being a target for histone and non-histone proteins, SIRT1 functions as a transcription factor for many different physiological processes (2).
What does SIRT1 stand for?
SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (Saccharomyces cerevisiae) is Sir2.
Do I need custom protocols for sirt1-substrate deacetylation in the presence of ex-527?
Time course of SIRT1-substrate deacetylation by recombinant SIRT1 in the presence of EX-527. To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Publishing research using ab141506?
How does Sirtuin 1 suppression alleviate airway inflammation?
Wu Y et al. Suppression of sirtuin 1 alleviates airway inflammation through mTOR-mediated autophagy. Mol Med Rep 22:2219-2226 (2020). PubMed: 32705226
Does Secukinumab attenuate reactive astrogliosis via IL-17RA/(C/ebpß/sirt1)?
Liu SP et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/ (C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019).