What is targeted gene transfer?
Gene targeting (also, replacement strategy based on homologous recombination) is a genetic technique that uses homologous recombination to modify an endogenous gene. The method can be used to delete a gene, remove exons, add a gene and modify individual base pairs (introduce point mutations).
How are targets selected for gene therapy?
Currently, targeting is achieved by engineering of the surface components of viruses and liposomes to achieve discrimination at the level of target cell recognition and/or by incorporating transcriptional elements into plasmid or viral genomes such that the therapeutic gene is expressed only in certain target cell …
How is gene transfer done?
It usually involves putting a gene or part of a gene obtained from one organism into the genome of a different organism. The alteration and recombination in the laboratory often involves cutting up DNA molecules and splicing together specific DNA fragments. The DNA may be natural or synthetic.
Which chemical is used for gene transfer methods?
Solution : Polyethylene Glycol (PEG) and Dextran Sulphate.
How do you choose a vector for gene therapy?
In conclusion, there are many kinds of vectors available for gene therapy, each with their own advantages and disadvantages. Which one to choose for your project comes down to factors like the size of the transgene, the kind of gene therapy target cells used, how the transgene should be expressed and more.
What techniques are used in gene therapy?
There are two different types of gene therapy depending on which types of cells are treated:
- Somatic gene therapy: transfer of a section of DNA to any cell of the body that doesn’t produce sperm or eggs.
- Germline gene therapy: transfer of a section of DNA to cells that produce eggs or sperm.
What is recombinant DNA technology PDF?
Recombinant DNA is the formation of a novel DNA sequence by the combination of two DNA fragments. It involves the method by which DNA of the donor organism (target DNA) is cut into fragments with the help of Restriction Enzymes and insert one of these fragments into the DNA of the host.
What technology is used in gene therapy?
Gene therapy is a therapeutic strategy using genetic engineering techniques to treat various diseases. In the early 1960s, gene therapy first progressed with the development of recombinant DNA (rDNA) technology,1) and was further developed using various genetic engineering tools, such as viral vectors.