What is the difference between deoxycholate and deoxycholic acid?
What is deoxycholic acid? Deoxycholic acid is a secondary bile acid produced by intestinal bacteria acting on primary bile acids secreted by the liver. Deoxycholic acid is also known as deoxycholate and cholanoic acid. Deoxycholic acid emulsifies fats to aid their intestinal absorption.
What is the function of deoxycholate?
Deoxycholic acid is a a bile acid which emulsifies and solubilizes dietary fats in the intestine, and when injected subcutaneously, it disrupts cell membranes in adipocytes and destroys fat cells in that tissue.
How do you make sodium deoxycholate solution?
The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light. The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml). Store the buffer at 4°C.
Is deoxycholate denatured?
Sodium cholate and sodium deoxycholate are the least denaturing of the commonly used ionic detergents. Two properties of detergents are important in their consideration for biological studies: the critical micelle concentration (CMC) and the micelle molecular weight (Table A. 1B.
Is deoxycholate a bile salt?
The human bile salt sodium deoxycholate induces metabolic and cell envelope changes in Salmonella Typhi leading to bile resistance.
How do you store deoxycholate?
Deoxycholate should be stored at room temperature and must be protected from light. Shelf life is limited and must be used used within a few days (not sure how long exactly but we used to use it all up within 48 hours of reconstitution as it starts precipitating).
What is the purpose of sodium deoxycholate in XLD media?
Principle of XLD Agar It utilizes sodium deoxycholate as the selective agent and, therefore, is inhibitory to gram-positive micro-organisms. Xylose is incorporated into the medium since it is fermented by practically all enterics except for the Shigella and this property enables the differentiation of Shigella species.
How do you dissolve deoxycholate?
Sodium deoxycholate precipitates in low pH. Ideally you should dissolve it in a solution buffered at slightly basic pH (~7.4-8). If this condition is not suitable for you always prepare a fresh solution in water and check/adjust the pH accordingly.
What is deoxycholate salt?
Sodium deoxycholate is a bile acid salt. It contains a deoxycholate. ChEBI. A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine.
How does sodium deoxycholate dissolve in water?
Is XLD media selective or differential?
XLD Agar is both a selective and differential medium. It contains a yeast extract as a source of nutrients and vitamins. It uses sodium deoxycholate as a selective agent and is therefore inhibitory to Gram-positive microorganisms.
What type of media is XLD agar?
Xylose Lysine Deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. The agar was developed by Welton Taylor in 1965.
How do you store sodium deoxycholate?
Can deoxycholic acid be used on belly fat?
A single session of DCA lipolysis can effectively reduce lower abdominal fat and even mild abdominal protuberance in thin patients. This minimally invasive technique is safe and well tolerated.
What grows black on XLD agar?
Some Proteus strains will give black-centered colonies on XLD Agar. For identification, organisms must be in pure culture. Morphological, biochemical, and/or serological tests should be performed for final identification.
What is the pH indicator of XLD agar?
indicator phenol red
The agar was developed by Welton Taylor in 1965. It has a pH of approximately 7.4, leaving it with a bright pink or red appearance due to the indicator phenol red.