What is TagRFP?
TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity.
How does the red fluorescence gene work?
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.
What is mRFP1?
mRFP1 is a basic (constitutively fluorescent) red fluorescent protein published in 2002, derived from Discosoma sp.. It is reported to be a somewhat slowly-maturing monomer with low acid sensitivity.
Why are proteins labeled with fluorescent molecules?
Fluorescent proteins are advantageous because there is no free dye in solution which can reduce the overall intensity changes observed with organic fluorophores. But, the large label size and limited choice of labeling sites prevent a targeted approach.
Where does the GFP gene come from?
Green fluorescent protein (GFP) was originally derived from the jellyfish Aequorea victoria (Prendergast and Mann, 1978). It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67.
How do you label fluorescent DNA?
Fluorescent labeling of nucleic acids is usually carried out by enzymatic reactions. Organic fluorophores are chemically introduced into primers or nucleoside triphosphates and are then incorporated either using PCR amplification or using DNA or RNA polymerases or terminal polynucleotide transferase ( 2–4 ).
How does the red fluorescent protein variant TagRFP-t improve photostability?
The red fluorescent protein variant TagRFP-T has greatly improved photostability over its parent molecule, TagRFP, but the underlying mechanism leading to this improvement is to date unknown. The 1.95 Å resolution crystallographic structure of TagRFP-T showed that its chromophore exists as a mixture …
Why is TagRFP-t more photostable than its parent molecule?
The crystal structure of red fluorescent protein TagRFP-T reveals the mechanism of its superior photostability The red fluorescent protein variant TagRFP-T has greatly improved photostability over its parent molecule, TagRFP, but the underlying mechanism leading to this improvement is to date unknown.
TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity.
How can I obtain DNA and protein sequence with restriction sites?
Monomeric derivative of red fluorescent protein from Entacmaea quadricolor. To obtain this DNA and protein sequence with restriction sites, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files. Don’t have either application? Download SnapGene or SnapGene Viewer.