What does the CMV promoter do?
The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.
What is ef1 promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
Does CMV promoter work in bacteria?
These results indicate that bacterial promoter activity, either within, or upstream of, the CMV promoter caused read-through transcription into the C-fragment gene resulting in bacterial expression.
What is TAC protein?
The Tac-Promoter (abbreviated as Ptac), or tac vector is a synthetically produced DNA promoter, produced from the combination of promoters from the trp and lac operons. It is commonly used for protein production in Escherichia coli.
What is minimal CMV promoter?
Minimal CMV promoter expression plasmid that allows the insertion of transcription factor binding sites upstream of the minimal promtoer to create promoters with novel functions such as responsiveness to transciption factors or drugs. Your gene of interest can be inserted into the MCS under control of the new promoter.
Does CMV promoter work in yeast?
We found that the CMV promoter in yeast cells is bidirectionally induced by osmotic stress and in glycerol media. The mitogen-activated protein (MAP) kinase Hog1 controls CMV activation by osmotic stress through the ATF/CRE-related transcription factor Sko1 and the yeast osmostress factor Hot1.
Why are lentiviruses good vectors for gene therapy?
Gene therapy vectors derived from lentiviruses offer many potentially unique advantages over more conventional retroviral gene delivery systems. Principal amongst these is their ability to provide long-term and stable gene expression and to infect non-dividing cells, such as neurons.
What is the difference between CMV and eEF1A1 promoter?
In comparison with the CMV promoter, the eukaryotic translation elongation factor 1 α (EF-1α, gene symbol EEF1A1) promoter increased the transfection efficiency, the transgene expression, the proportion of expression-positive clones and the copy number of the episomal vector in long-term culture.
Do synthetic CMV core-ef1-α hybrid promoters improve transgene expression?
The synthetic CMV core-EF1-α hybrid promoter can significantly improve transgene expression compared with the control (EF1-α promoter), with a maximum ten-fold increase, in CHO cells ( Magnusson et al., 2011 ).
Is the EF‐1α promoter a potent regulatory sequence for episomal vectors?
Our results revealed that the EF‐1α promoter is a potent regulatory sequence for episomal vectors and maintains high transgene expression. Plasmids containing the EF‐1α promoter were found to replicate efficiently, stably and extrachromosomally in CHO‐K1 cells.
Is the EF‐1α promoter stronger than the CAG promoter during long‐term culture?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells. However, in the present study, the CAG promoter could not maintain transgene expression stably during long‐term culture as compared with the CMV promoter.