How do you make 1000X chloramphenicol stock solution?
Directions:
- Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol.
- Filter through a 0.22 µm filter to sterilize.
- Aliquot and store at -20°C.
- Use at 1:1000 dilution in LB or LB-Agar.
How do you make 50 mg mL of chloramphenicol?
Add 10 ml of 95% EtOH (70% may also be used).
How do you make chloramphenicol 34mg mL?
We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml of 70% ethanol and mix the content by rotating the tube. Once the content is mixed, make up the volume to 10 ml.
What do you dilute chloramphenicol in?
Dosage and Administration The following method of administration is recommended: Intravenously as a 10% (100 mg/mL) solution to be injected over at least a one-minute interval. This is prepared by the addition of 10 mL of an aqueous diluent such as water for injection or 5% dextrose injection.
How much chloramphenicol do I add to LB broth?
Chloramphenicol – The frozen stock solutions of chloramphenicol are at 25mg/ml in 100% ethanol and are marked with purple. The final concentration for LB liquid culture is 25mg/ml. To obtain this in 100ml of LB, add 100ul stock solution.
Does chloramphenicol dissolve in water?
Solubility : Soluble in water (2.5 g/l) at 25° C, methanol, ethanol, butanol, ethyl acetate, acetone, DMSO, and propylene glycol (150.8 mg/ml).
How do I make a 50ml solution?
To prepare 50 mg/mL BCIP disodium salt, dissolve 0.5 g in 10 mL H2O.
Can you dissolve chloramphenicol in water?
In purified water the solubility of chloramphenicol is only 0.25%.
How do you calculate antibiotic stock solution?
Make sure units match!
- C1V1 = C2V.
- C = concentration; V = volume.
- Example: You have a stock solution of A at 100mg/ml. You want 150 ml at 100ug/ml.
- (100ug/ml)(150ml) = (100000ug/ml)(x)
- 15000 = 100000x.
- x = 0.15ml, or 150ul.
- Remember that you have to convert mg/ml to ug/ml, which you can do by multiplying by 1000.
How do you store chloramphenicol powder?
It is suggested the stock solution should be stored refrigerated and used within 30 days.
How do you prepare a stock solution?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure’s the reagent depends on the desired concentration unit.
How do you make a stock solution of antibiotics?
For a stock solution of 10 mg/mL:
- 200 mg Erythromycin.
- 20 mL 100% ethanol (in fume hood)
- Do not filter sterilize—the ethanol may dissolve the filter.
How much chloramphenicol do I add to PDA?
You can add chloramphenicol with an average of 0.05 gm. l-1 to PDA medium before autoclaving because the chlramphenicol is not be affected by heating and then put the medium in autoclave at 121 0C and 15 pound. inch -1 for 20-30 minutes.
Can chloramphenicol dissolve in water?
How do you make a 100 mM stock solution?
For practical purpuse it is best to prepare a concentrated stock solution. 416.6 mg in 10 ml will give you 100 mM solution. This can be aliquoted into multiple vials and kept frozen. When needed, thaw one vial and then use this stock soluition to make further dilutions.
What is standard stock solution?
A standard solution or a stock solution is a solution whose concentration is accurately known. 2. At the time of experiment, the solution with required concentration is prepared by diluting the stock solution. This diluted solution is called working standard.
How do you make 34 mg of chloramphenicol in ethanol?
Chloramphenicol Chloramphenicol – 10 mL (1,000 X Stock) 34 mg/ml chloramphenicol in ethanol Reagents needed: 0.34 g chloramphenicol 10 ml 100% ethanol Directions: 1) Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol.
How to prepare chloramphenicol sulfate solution?
1 Weight 0.85g of chloramphenicol sulfate into a small weight boat. 2 Add 25ml 100% EtOH to a 50ml Falcon tube and add chloramphenicol 3 Mix/vortex vigorously so all the chloramphenicol goes into solution. 4 Store at -20C.
What is the mechanism of action of chloramphenicol in bacterial culture?
Store at -20C. Antibiotic Mode of Action: Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of “amplifying” plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.