Table of Contents
What is Acid Fuchsin stain used for?
Acid fuchsin has wide use in histology, and is one of the dyes used in Masson’s trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen.
How do you make basic fuchsin stain?
Dissolve 0.5 g basic fuchsin dye in 20 ml 95% ethanol. Dilute to 100 ml with distilled water. Filter if necessary with Whatman No. 31 filter paper to remove any undissolved dye.
Is Acid Fuchsin a basic stain?
Acid fuchsin has wide use in histology, and is one of the dyes used in Masson’s trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen….CHEBI:87052 – acid fuchsin.
| ChEBI Name | acid fuchsin |
|---|---|
| ChEBI ID | CHEBI:87052 |
How do you stain fungi with Lactophenol cotton blue?
Immerse the specimen/material in the drop of alcohol. Add one, or at most two drops of the lactophenol/cotton blue mountant/stain before the alcohol dries out. Holding the coverslip between forefinger and thumb, touch one edge of the drop of mountant with the coverslip edge, and lower gently, avoiding air bubbles.
What does fuchsin mean?
Definition of fuchsin : a dye that is produced by oxidation of a mixture of aniline and toluidines and yields a brilliant bluish red.
What color is basic fuchsin?
Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl.
Is there any alternative for lactophenol cotton blue?
Iodine-glycerol as an alternative to lactophenol cotton blue for identification of fungal elements in clinical laboratory.
What is Fuchsin reagent?
Fuchsin Acid reagent is used as a component of the Mallory Trichrome kit. Mallory trichrome staining kit is used for treating the tested microscopic sample using three different stainings with differential counterstaining of two basic parts of the tissue (muscle and collagen fibers) in focus.
Why is Fuchsin magenta?
It becomes magenta when dissolved in water; as a solid, it forms dark green crystals. As well as dying textiles, fuchsine is used to stain bacteria and sometimes as a disinfectant….Fuchsine.
| Names | |
|---|---|
| ChEBI | CHEBI:87665 |
| ChEMBL | ChEMBL1979636 |
| ChemSpider | 10468578 |
| ECHA InfoCard | 100.010.173 |
What does aldehyde fuchsin stain?
al·de·hyde fuch·sin. a stain using basic fuchsin paraldehyde and hydrochloric acid; it produces violet staining of elastic fibers, mast cell granules, gastric chief cells, β cells of the pancreatic islets, and certain hypophyseal β granules; other pituitary granules and cells stain in other colors.
Why is fuchsin magenta?
How do you make cotton blue Lactophenol?
A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:
- Distilled water 50ml.
- Cotton Blue (Aniline Blue) 0.125g.
- Phenol Crystals (C6H5O4) 50g.
- Glycerol 100ml.
- Lactic acid (CH3CHOH COOH) 50ml.
- 70% ethanol.
How do you make iodine-glycerol solution?
A iodine-glycerol solution prepared by a take 1.0 gm Lugols Iodine and 2.0 gm Potassium Iodine and dissolve into 100 ml distilled water, mix well and keep in a brown reagent bottle [10] Evaluation of Iodine-Glycerol for Wet-Mount Preparation of Feces for Detection of Intestinal Parasites.
Why is lactophenol used for mold?
Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.
Why is Lactophenol blue used?
Lactophenol blue solution is used to visualise fungal cells in various cytological materials.
What is iodine glycerol used for?
Iodinated glycerol is used as a mucolytic agent in respiratory disorders. Organically bound iodine is changed to unbound iodide after absorption. Iodide inhibits the binding of iodine to the tyrosine residue of the thyroglobulin molecule, inhibiting the synthesis of thyroxine and triiodothyronine.