Table of Contents
What is the size of range of T-DNA?
The length of the mobilized T-strand, when coated with VirE2 molecules, is estimated to range between 40 nm and 80 microns for T-DNA regions between 20 Kb and 150 Kb.
What is the size range of T-DNA in KB?
between 15 and 30 kb
What is the size range of T-DNA? Explanation: The size range of T-DNA is between 15 and 30 kb depending upon the strain of bacteria. The whole of this region is integrated into a host chromosome.
What is the length of Ti plasmid?
Octopine-type Ti plasmids carry two T-DNA fragments designated TL-DNA and TR-DNA of 13 and 7.8 kb in length, respectively (see Fig.
What is the full form of T-DNA?
The native transfer DNA (T-DNA) carries a set of oncogenes (Gaudin et al., 1994) and opine catabolism genes, whose expression in plant cells leads to neoplastic growth of the transformed tissue and the production of opines, amino acid derivatives that are used by the bacteria as a nitrogen source.
How do I choose A transformant?
Transformants are selected from non-transformants by the presence of a selectable marker, e.g. transformants can be selected by plating them on the antibiotic-containing medium, for the antibiotic resistance gene present in the plasmid.
What is the size of Ti plasmid Mcq?
The size of the Ti plasmid is more than 200 kb in size that contains the genes involved in the infective process. Explanation: The remarkable feature of Ti plasmid is that, after infection, a part of the molecule is incorporated into the plant genome. This segment of the Ti plasmid is called the T-DNA or transfer DNA.
Is transformants and recombinant the same?
Transformants vs Recombinants Transformants are cells that are introduced with the recombinant DNA molecule. Recombinants are carrier molecules that have foreign DNA inserted into their genome. They are cells that are able to express recombinant DNA. They are able to self replicate within the host organism.
What is transformants and non-transformants?
Transformants are the cells that have taken up the foreign DNA by transformation, whereas non-transformants are the cells that have not undergone a transformation.
Is T-DNA double stranded?
The T-DNA, defined by two 25-bp direct-repeat border sequences, travels as a single-stranded (ss) DNA molecule (T-strand), with a single Agrobacterium VirD2 protein attached to its 5′ end1,2,3.
What is the difference between Ti plasmid and Ri plasmid?
Ti plasmid is found in the bacterium Agrobacterium tumefaciens. Ri plasmid is found in the bacterium Agrobacterium rhizogenes. They cause crown gall disease in dicots. They cause hairy root disease in monocots.
How do you inject DNA into a plant cell?
DNA or RNA can be directly introduced into plant cells via a so-called microprojectile bombardment method, also known as a gene gun or biolistics. In this method, the transgene is coated onto microgold or tungsten particles and fired into plant cells ballistically [6].