Table of Contents
What special staining technique is used to positively identify iron?
Perl’s iron stain is the classic method for demonstrating iron in tissues. The section is treated with dilute hydrochloric acid to release ferric ions from binding proteins. These ions then react with potassium ferrocyanide to produce an insoluble blue compound (the Prussian blue reaction).
What is the stain for lipids?
The staining of lipids is generally called “fat staining.” Lipids include glycerides, phospholipids, glycolipids, and fatty acids. While various lipid stains show slightly different color tones, they all exhibit similar stainability.
What does eosin stain?
Eosin can be used to stain cytoplasm, red blood cells, collagen, and muscle fibers for histological examination. It is most often used as a counterstain to hematoxylin in H&E staining.
How does Prussian blue stain work?
Prussian blue reaction involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (+3) present in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called Prussian blue, or ferric ferrocyanide.
What does Gram stain stain?
The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
What are the oil soluble dyes used in lipid staining?
Oil red O is a diazo dye which has been widely used for staining fat cell and lipids since long ago. This lysochrome (fat-soluble dye) red dye can be used for staining of neutral triglycerides and low polar lipids.
Why does the nucleus stain blue?
Haematoxylin in complex with aluminium salts is cationic and acts as a basic dye. It is positively charged and can react with negatively charged, basophilic cell components, such as nucleic acids in the nucleus. These stain blue as a result.
What Colour does eosin stain?
pink
Eosin is pink and stains proteins nonspecifically. In a typical tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining.
How does silver nitrate stain work?
After repeated washing with water, the gel is incubated in a silver nitrate solution. Silver ions bind to negatively charged side chains of the proteins. Excess silver ions are then washed off with water.
Why does silver nitrate stain?
The black stain happened due to the fact this compound decomposition of the Ag+ ion in the body and turn it into Ag, later if come in contact with silver nitrate. Silver nitrate will absorbed by the skin and slowly experience decomposing with the silver ion element and reverting it into silver.
Can you survive myelofibrosis?
The only curative treatment for myelofibrosis (MF) continues to be allogeneic hematopoietic stem cell transplant (HSCT). Occasionally, adverse events posttransplantation can occur and usually present within the first 2 years after posttransplant.
Why is the colour of silver nitrate blue in colour?
Copper being more reactive than silver displaces silver from silver nitrate solution and forms copper nitrate that is blue in colour. Also a layer of silver is deposited on copper which makes it look silvery.
What is the reaction between silver nitrate and copper?
Silver nitrate is a clear and colourless solution, as the reaction occurs you will observe a change in colour of the solution from colourless to clear blue. Aqueous Cu^2+ ions make a solution blue. On the surface of the copper turnings a soft, silver/grey coating starts to form.
What happens when copper displace silver from aqueous solution?
In other words, copper displaces silver and forms a blue coloured aqueous solution of copper nitrate. (a) Silver nitrate solution is kept in coloured bottles.
What is this coating on the surface of the copper turnings?
On the surface of the copper turnings a soft, silver/grey coating starts to form. These are silver crystals.