What is QG buffer?
Buffer QG is a solubilization and binding buffer (with pH indicator), for use in DNA cleanup procedures. Buffer PE is a wash buffer for use in DNA cleanup procedures. It is supplied as a 5x concentrate of 100 ml, providing a final volume of 500 ml of buffer.
What is in Qiagen PB buffer?
Buffer PB contains a high concentration of guanidine hydrochloride and isopropanol. The exact composition of Buffer PB is confidential.
What is the composition of buffer aw1?
Buffer RW1 contains a guanidine salt, as well as ethanol, and is used as a stringent washing buffer that efficiently removes biomolecules such as carbohydrates, proteins, fatty acids etc., that are non-specifically bound to the silica membrane.
How does a Qiagen spin-column work?
QIAprep spin columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer.
What industry is QIAGEN in?
It serves molecular diagnostics, academia, pharmaceutical, and applied testing customers.
What is the composition of QIAGEN buffer QC?
– QIAGEN What is the composition of buffer QC? Buffer QC is the wash buffer used in QIAGEN Plasmid Kits for plasmid purification and in QIAGEN Blood & Cell Culture kits. The composition of Buffer QC is: 50 mM MOPS, pH 7.0
What is the difference between buffer PB and QG?
Buffer PB is used in DNA cleanup procedures and enables efficient binding of single- or double-stranded PCR products to the spin-column membrane. Buffer QG is a solubilization and binding buffer (with pH indicator), for use in DNA cleanup procedures.
What is the pH of QIAGEN buffer P3?
Buffer P3 (not for spin columns, but for Qiatips, midi, maxi, giga kits) 3.0 M potassium acetate pH 5.5 Buffer DP3 (for Qiagen Directprep 96-well miniprep) 3.0 M ammonium acetate pH 5.5 pH 4.8 20 mM Tris HCl pH 6.6 10 mM Tris-HCl pH 7.5 Buffer QX1 (for solution and binding of agarose gels) pH 5.3
What is the wash buffer in QIAGEN plasmid kits?
Buffer QC is the wash buffer used in QIAGEN Plasmid Kits for plasmid purification and in QIAGEN Blood & Cell Culture kits. The composition of Buffer QC is: