Table of Contents
What enzymes are involved in transcription?
RNA polymerase
Transcription is carried out by an enzyme called RNA polymerase and a number of accessory proteins called transcription factors. Transcription factors can bind to specific DNA sequences called enhancer and promoter sequences in order to recruit RNA polymerase to an appropriate transcription site.
What does post-transcriptional modification include?
Post-transcriptional modification includes the processes of polyadenylation, 5′ capping, and splicing. Polyadenylation is the addition of adenine bases (the poly-A tail) to the 3′ end of the RNA primary transcript. The poly-A tail is important in export, stability, and translation of the transcript.
Which is the first enzyme in capping?
The capping reaction is catalyzed by three enzymes: (1) RNA triphosphatase, which removes the terminal phosphate; (2) RNA guanylyltransferase, which transfers GMP from GTP to the diphosphate end of RNA to form the GpppN cap; and (3) RNA (guanine-7)-methyltransferase, which adds a methyl group to the N7 position of the …
What enzyme is primarily involved in translation?
– Translocase (EF-G) – elongation factor that utilizes GTP.
What enzyme initiates the process of transcription?
RNA polymerase is the main transcription enzyme. Transcription begins when RNA polymerase binds to a promoter sequence near the beginning of a gene (directly or through helper proteins). RNA polymerase uses one of the DNA strands (the template strand) as a template to make a new, complementary RNA molecule.
Which set of enzymes are part of 5 capping process?
Which enzyme is required for capping?
Capping is a three-step process that utilizes the enzymes RNA triphosphatase, guanylyltransferase, and methyltransferase.
What is the enzyme that catalyzes RNA synthesis?
enzyme RNA polymerase
The enzyme RNA polymerase catalyzes the chemical reactions that synthesize RNA, using the gene’s DNA as a template.
What two enzymes are involved in transcription and what roles do they play?
During transcription, RNA polymerase makes a copy of a gene from the DNA to mRNA as needed. During transcription, the DNA site at which RNA polymerase binds is called as a promoter. Transcription commences with one or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.
Which enzyme is responsible for the synthesis of mRNA?
mRNA is synthesized in the nucleus using the nucleotide sequence of DNA as a template. This process requires nucleotide triphosphates as substrates and is catalyzed by the enzyme RNA polymerase II. The process of making mRNA from DNA is called transcription, and it occurs in the nucleus.
What enzyme catalyzes the attachment of an amino acid to tRNA?
Aminoacyl-tRNA synthetases (aaRSs) are universally distributed enzymes that catalyze the esterification of a tRNA to its cognate amino acid (i.e., the amino acid corresponding to the anticodon triplet of the tRNA according to the genetic code) (Ibba and Soll 2000; Pang et al. 2014).
Which of the enzymes is involved in capping mRNA?