Table of Contents
What is the colour of Janus Green B solution?
The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
What does Janus Green B stain?
What is Janus Green B? Janus Green B is a dark green to black powder belonging to the phenazine group of dyes. Introduced by Leonor Michaelis in 1900, the interesting feature of this dye is that it must be oxidised to become coloured. In histology, this basic dye is used to stain mitochondria in living tissues.
Why does Janus green identify mitochondria?
(d) Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present. It oxidizes to blue colour in. presence of oxygen and in its absence changes its colour to pink.
How do you prepare Janus Green B solution?
Solution Prep To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.
What is Janus Green B solution?
A working solution of 0.02% Janus Green B in saline is briefly applied to the exposed peripheral nervous system. The stain is then decanted and the dissection flooded with fresh saline, resulting in whole nerves being stained dark blue in contrast to surrounding tissues.
What is the role of Janus Green B stain in the slide preparation?
The Janus Green B is a stain which acts as a indicator. It changes it’s color due to the presence of oxygen. It oxidizes the blue color otherwise in absence the color becomes pink. The indicator is used to stain the mitochondria in the living cell.
What is role of Janus Green B stain in this slide preparation?
Answer: Janus green is used to stain exclusively mitochondria. If this stain is not used, mitochondria can’t be distinguished from other cellular organelles.
Why do we stain organisms?
Why Stain Cells? The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Which color will the nucleus stain during microscopic observation?
Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.
What is the meaning of Janus green?
Definition of Janus green : a basic azine dye used especially as a vital biological stain (as for mitochondria)
Which color is blue in nucleus?
Safranin is a nuclear stain used as a counterstain and it can also be used to color collagen yellow. Nile blue used in living cells to stain nucleus blue.
Why does nucleus appear deep blue after staining with methylene blue?
Methylene Blue is a popular alkaline stain used to stain animal cells to make nuclei more visible. Methylene blue binds DNA which is very abundant in the nucleus. Cells stained by methylene blue will show the nucleus with a deep blue color.
Is methylene blue positive or negative staining?
Notes: Methylene Blue is a cationic stain (positively charged blue dye); and binds to negatively charged parts of the cells, such as nucleus (DNA) and RNA in the cytoplasm (with lower affinity).
What is the stability of carbol fuchsin?
Carbol fuchsin ——– 6 months. 2. *Acid alcohol ——– 6 months. 3. Methylene blue ——– 6 months.
Why is Janus Green B used to stain mitochondria?
It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour.
What is the colour of Janus Green B indicator?
The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
What does Janus Green B do for lymphatic vessels?
With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells. 1. Yack, J.E. 1993. J. Neurosci. Methods. 49: 17-22.
What is Janus Green B chloride?
Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation. 8- [ [4- (dimethylamino)phenyl]diazenyl]- N, N -diethyl-10-phenylphenazin-10-ium-2-amine;chloride