Table of Contents
What reaction to feulgen stain is made by DNA?
The principle of Feulgen stain is to dissociate the two strands of DNA through hydrolysis by a solution of molar HCl which destroys the purine bases. HCl separates the two purine bases of DNA: adenine, guanine, liberating the hemiacetal functions of deoxyriboses.
What is Feulgen-positive and negative?
SUMMARY. The nuclei of the male and female gametocytes of the malarial parasites {Plasmo- dium and Hepatocystis) are Feulgen-negative, while the nuclei of the gametocytes of Hepatozoon, which are not sexually differentiated, are Feulgen-positive.
How does Schiff’s reagent stain the DNA?
Schiff’s reagent is the stain used in this technique. It specifically stains the DNA due to reaction of Schiff’s (or Schiff’s-like) reagent with aldehyde groups exposed at C1 as the result of the nitrogen bases cleavage from deoxyribose by 1N HCl hydrolysis at 60°C within about 10 minutes.
Which of the following color has taken by DNA after staining using Feulgen technique?
DNA should be stained red. The background, if counterstained, is green. The Feulgen reaction is a semi-quantitative technique.
Which bond is cleaved by acid hydrolysis during Feulgen staining of DNA?
Diethylpyrocarbonate (Figure 6) reacts with adenine and guanine bases in nucleic acids resulting in rupture of the purine ring and subsequent cleavage of the glycosidic bond.
What is acid hydrolysis of DNA?
Acidic hydrolysis of deoxyribonucleic acid (DNA) constitutes the usual. way to obtain the quantitative release of the free purine and pyrimidine. nucleobases. One of the most convenient approach involves the use of. concentrated formic acid at high temperature (180’C) for 30 minutes to induce.
What is hydrolysis of DNA?
Hydrolysis of the DNA backbone is an important reaction in biology and in the laboratory manipulation of genetic material. While many enzymes catalyze the sequence-selective hydrolysis of DNA, it has been difficult to design nonprotein catalysts for this purpose.
What happens when DNA undergoes hydrolysis?
Complete hydrolysis of chromosomal nucleic acids gave inorganic phosphate, 2-deoxyribose (a previously unknown sugar) and four different heterocyclic bases (shown in the following diagram). To reflect the unusual sugar component, chromosomal nucleic acids are called deoxyribonucleic acids, abbreviated DNA.
What causes DNA hydrolysis?
Hydrolytic damage may result from the biochemical reactions of various metabolites as well as the overabundance of reactive oxygen species. Ultraviolet and other types of radiation can damage DNA in the form of DNA strand breaks.
What is the Feulgen reaction for DNA?
The Feulgen reaction for DNA was initially described by Feulgen and Rossenbeck in 1924 (42). They found that a mild acid hydrolysis of fixed tissue sections followed by treatment with Schiff’s reagent (43) resulted in a dis- Hydrolysis Times in 5 N HCl for Invertebrate Tissues Prepared in Different Fixatives
What are the applications of Feulgen reaction?
The Feulgen reaction has been proposed by Robert Feulgen and Heinrich Rossenbeck for the identification of DNA nearly a hundred years ago. Since then, many other applications of this cytochemical/topochemical procedure at qualitative and quantitative level have been proposed in relation to DNA and i …
Do Feulgen reaction DNA stain reagents expire?
Feulgen Reaction DNA Stain Reagents for this procedure are sold as individual stain solutions and are available for purchase under separate part numbers with storage requirements and expiration date designated per bottle.
What is newcomer supply Feulgen reaction?
The Newcomer Supply Feulgen Reaction procedure is for the demonstration of DNA (deoxyribonucleic acid) in tissue sections. Fixation: Formalin 10%, Phosphate Buffered ( Part 1090) See Procedure Note #1. Solutions: All solutions are manufactured by Newcomer Supply, Inc.