What is the fractionation range of Sephadex G-75?
Select your model
| Parameter | Sephadex G-75, 100 g |
|---|---|
| Fractionation range (Mr) globular proteins 1 | 4 × 10³–5 × 10⁴ |
| Matrix | Cross-linked dextran |
| PSDdry (within specification) 2 | 40 µm – 120 µm |
| PSDwet 3 | 92 µm – 293 µm (40 µm – 320 µm) |
What is the role of Sephadex in protein purification?
Applications. Sephadex is used to separate molecules by molecular weight. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries.
How does Sephadex G-25 work?
Sephadex G-25 resins and prepacked columns offer: Large molecules are excluded while smaller sized molecules enter the beads to varying extents according to their different sizes. Large molecules thus leave the column first, followed by smaller molecules.
What is Sephadex g10?
Sephadex® G-10 is a gel filtration medium used in gel filtration chromatography and protein chromatography. Sephadex® G-10 has been used in cancer studies and to determine fluoride levels in tap water, river water and green tea samples.
What does Sephadex G-100 mean?
Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography. It has been used: in gel filtration column for α-amylase purification.
What is the fractionation range for Sephadex G-100?
Select Model
| Parameter | Sephadex G-100, 100 g |
|---|---|
| Fractionation range (Mr) globular proteins 1 | 5 × 10³ – 6 × 10⁵ |
| Matrix | Cross-linked dextran |
| PSDdry (within specification) 2 | 40 µm – 120 µm |
| PSDwet 3 | 94 µm – 329 µm (40 µm – 360 µm) |
What is the exclusion limit of Sephadex G-25?
approximately Mr 5000
Sephadex G-25 has a fractionation range for globular proteins of molecular weights (Mr) 1000 to 5000, with an exclusion limit of approximately Mr 5000.
What is Sephadex in column chromatography?
Sephadex is a cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin. The name is derived from separation Pharmacia dextran. It is normally manufactured in a bead form and most commonly used for gel filtration columns.