What is Rotor Gene Q?
QIAGEN’s real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN® kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.
How does a thermal cycler help with the process of PCR?
A thermocycler or PCR machine is a laboratory apparatus used for PCR. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then rises and lowers the temperature of the block in discrete, pre-programmed steps.
What is the principle of thermal cycler?
The Thermal Cycler (also known as a Thermocycler, PCR Machine or DNA Amplifier) is a laboratory apparatus used to amplify segments of DNA via the Polymerase Chain Reaction (PCR). The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted.
What is the principle of PCR test?
Principle of PCR PCR uses the enzyme DNA polymerase that directs the synthesis of DNA from deoxynucleotide substrates on a single-stranded DNA template. DNA polymerase adds nucleotides to the 3` end of a custom-designed oligonucleotide when it is annealed to a longer template DNA.
Can you have Covid and test negative PCR?
If you were exposed to COVID (e.g., someone you live with has it), and you have symptoms, but test negative on an antigen or PCR test, here are some possible explanations for what may be happening: 1. It’s too early, and your viral load may not be high enough to show up on a test.
What is QIAGEN Rotor Gene Q used for?
QIAGEN Rotor-Gene Q (Corbett Rotor-Gene 6000) — Pure Detection. The Rotor-Gene Q is an innovative real-time cycler that enables high-precision real-time PCR thanks to its unique rotary design.
What makes the Rotor-Gene Q the best real time PCR cycler?
The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure “Cross-section of the Rotor-Gene Q”). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling.
Is the investigator Quantiplex kit validated for the Rotor-Gene Q?
The Investigator Quantiplex Kit has been validated for the Rotor-Gene Q, and the Applied Biosystems 7500 Fast and 7500 Real-Time PCR Systems. How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
Can data be analyzed on the Rotor-Gene Q while it is running?
Yes, data can be analyzed on the Rotor-Gene Q while a run is in progress, allowing to save time for planning and setting up new experiments. Multiple copies of the Rotor-Gene Q software can be opened simultaneously during a run allowing analysis of previous experiments while the system is running. What is a QuantiTect Primer Assay?